Research Institute of Pharmaceutical Sciences, Musashino University, 1-1-20 Shinmachi, Nishitokyo-shi, Tokyo 202-8585, Japan.
Department of Pharmacy, Ogikubo Hospital, 3-1-24 Imagawa, Suginami-ku, Tokyo 167-0035, Japan.
Nutrients. 2021 Sep 21;13(9):3297. doi: 10.3390/nu13093297.
We have previously shown that two enteral nutrition formulas suppressed gastric lesions induced by the oral administration of indomethacin (IND) in mice. However, the mechanism of their protective effect is unknown. In this study, the effect of the two enteral nutrition formulas on gastric lesions induced by subcutaneous IND injection was investigated, with the objective of exploring the possibility that they may interact directly with IND in the gastrointestinal tract. Ten-week-old, male, ICR mice were fasted, then orally given either purified water, Mermed One, or 2-fold diluted Terumeal 2.0α as enteral nutrition formula (25 mL/kg). IND was injected subcutaneously at 20 mg/kg after 30 min, and the stomach was removed 6 h later and fixed in formalin. The number and area of lesions in the stomachs of mice given enteral nutrition formula was reduced to 56-89% and 34-61%, respectively, compared with the mice given purified water. The time courses of plasma IND concentrations were comparable among the three groups. These results suggested that the effect of these enteral nutrition formulas on gastric lesions did not originate from their direct interaction with IND in the gastrointestinal tract or their effect on the disposition of IND.
我们之前已经证明，两种肠内营养配方可抑制小鼠口服吲哚美辛（IND）引起的胃损伤。然而，其保护作用的机制尚不清楚。在这项研究中，我们研究了这两种肠内营养配方对皮下注射 IND 诱导的胃损伤的影响，旨在探讨它们是否可能在胃肠道内与 IND 直接相互作用。将 10 周龄雄性 ICR 小鼠禁食，然后分别口服给予纯净水、Mermed One 或 2 倍稀释的 Terumeal 2.0α 作为肠内营养配方（25 mL/kg）。30 分钟后，经皮下给予 IND 20mg/kg，6 小时后取出胃并固定在福尔马林中。与给予纯净水的小鼠相比，给予肠内营养配方的小鼠的胃损伤数量和面积分别减少了 56-89%和 34-61%。三组小鼠的血浆 IND 浓度的时间过程相似。这些结果表明，这些肠内营养配方对胃损伤的作用并非源于它们与 IND 在胃肠道中的直接相互作用，也不是源于它们对 IND 处置的影响。
