Kovacs Zoltan, Muncan Jelena, Ohmido Nobuko, Bazar George, Tsenkova Roumiana
Department of Measurements and Process Control, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Somlói út 14-16, 1118 Budapest, Hungary.
Biomeasurement Technology Laboratory, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Hyogo, Japan.
Plants (Basel). 2021 Sep 3;10(9):1832. doi: 10.3390/plants10091832.
In vivo monitoring of rice ( L.) seed germination and seedling growth under general conditions in closed Petri dishes containing agar base medium at room temperature (temperature = 24.5 ± 1 °C, relative humidity = 76 ± 7% (average ± standard deviation)), and induced degenerated callus formation with plant growth regulator, were performed using short-wavelength near-infrared spectroscopy and aquaphotomics over A period of 26 days. The results of spectral analysis suggest changes in water absorbances due to the production of common metabolites, as well as increases in biomass and the sizes of the samples. Quantitative models built to predict the day of the development provided better accuracy for rice seedlings growth compared to callus formation. Eight common water bands were identified as presenting prominent changes in the absorbance pattern. The water matrix of only rice seedlings showed three developmental stages: firstly expressing a predominantly weakly hydrogen-bonded state, then a more strongly hydrogen-bonded state, and then, again, a weakly hydrogen-bonded state at the end. In rice callus induction and proliferation, no similar change in water absorbance pattern was observed. The presented findings indicate the potential of aquaphotomics for the in vivo detection of degeneration in cell development.
在室温（温度 = 24.5 ± 1 °C，相对湿度 = 76 ± 7%（平均值 ± 标准差））下，在含有琼脂基础培养基的封闭培养皿中，对水稻（L.）种子在一般条件下的萌发和幼苗生长进行体内监测，并使用植物生长调节剂诱导退化愈伤组织形成，通过短波近红外光谱和水相光组学技术进行了为期26天的监测。光谱分析结果表明，由于常见代谢物的产生，水吸收率发生了变化，同时样品的生物量和尺寸也有所增加。与愈伤组织形成相比，为预测发育天数建立的定量模型对水稻幼苗生长的预测准确性更高。确定了八个常见的水吸收带，其吸光度模式呈现出显著变化。仅水稻幼苗的水基质显示出三个发育阶段：首先主要表现为弱氢键结合状态，然后是更强的氢键结合状态，最后又回到弱氢键结合状态。在水稻愈伤组织诱导和增殖过程中，未观察到水吸收率模式的类似变化。所呈现的研究结果表明水相光组学在体内检测细胞发育退化方面具有潜力。
