ω-3 脂肪酸对 NCAA 橄榄球运动员头部创伤生物标志物的影响：一项多中心、非随机研究。
The effect of omega-3 fatty acids on a biomarker of head trauma in NCAA football athletes: a multi-site, non-randomized study.
机构信息
Department of Health, Human Performance, and Recreation, Baylor University, Waco, TX, USA.
Wake Forest School of Medicine, Winston-Salem, NC, USA.
出版信息
J Int Soc Sports Nutr. 2021 Sep 27;18(1):65. doi: 10.1186/s12970-021-00461-1.
BACKGROUND
American-style football (ASF) athletes are at risk for cardiovascular disease (CVD) and exhibit elevated levels of serum neurofilament light (Nf-L), a biomarker of axonal injury that is associated with repetitive head impact exposure over the course of a season of competition. Supplementation with the w-3 fatty acid (FA) docosahexaenoic acid (DHA) attenuates serum Nf-L elevations and improves aspects of CVD, such as the omega-3 index (O3I). However, the effect of combining the w-3 FA eicosapentaenoic acid (EPA) and docosapentaenoic acid (DPA) with DHA on, specifically, serum Nf-L in ASF athletes is unknown. Therefore, this study assessed the effect of supplemental w-3 FA (EPA+DPA+DHA) on serum Nf-L, plasma w-3 FAs, the O3I, and surrogate markers of inflammation over the course of a season.
METHODS
A multi-site, non-randomized design, utilizing two American football teams was employed. One team (n = 3 1) received supplementation with a highly bioavailablew-3 FA formulation (2000mg DHA, 560mg EPA, 320mg DPA, Mindset®, Struct Nutrition, Missoula, MT) during pre-season and throughout the regular season, while the second team served as the control (n = 35) and did not undergo supplementation. Blood was sampled at specific times throughout pre- and regular season coincident w ith changes in intensity, physical contact, and changes in the incidence and severity of head impacts. Group differences were determined via a mixed-model between-within subjects ANOVA. Effect sizes were calculated using Cohen's dfor all between-group differences. Significance was set a priori at p< .05.
RESULTS
Compared to the control group, ASF athletes in the treatment group experienced large increases in plasma EPA (p < .001, d = 1.71) and DHA (p < .001, d = 2.10) which contributed to increases in the O3I (p < .001, d = 2.16) and the EPA:AA ratio (p = .001, d = 0.83) and a reduction in the w-6: w-3 ratio (p < .001, d = 1.80). w-3 FA supplementation attenuated elevations in Nf-L (p = .024). The control group experienced a significant increase in Nf-L compared to baseline at several measurement time points (T2, T3, and T4 [p range < .001 - .005, drange = 0.59-0.85]).
CONCLUSIONS
These findings suggest a cardio- and neuroprotective effect of combined EPA+DPA+DHA w-3 FA supplementation in American-style football athletes.
TRIAL REGISTRATION
This trial was registered with the ISRCTN registry ( ISRCTN90306741 ).
背景
美式足球运动员存在心血管疾病（CVD）风险，其血清神经丝轻链（Nf-L）水平升高，Nf-L 是一种轴突损伤的生物标志物，与整个赛季的反复头部撞击暴露有关。ω-3 脂肪酸（FA）二十二碳六烯酸（DHA）的补充可降低血清 Nf-L 升高，并改善 CVD 等方面，如 ω-3 指数（O3I）。然而，联合 ω-3 FA 二十碳五烯酸（EPA）和二十二碳五烯酸（DPA）与 DHA 对美式足球运动员血清 Nf-L 的具体影响尚不清楚。因此，本研究评估了补充 ω-3 FA（EPA+DPA+DHA）对血清 Nf-L、血浆 ω-3 FA、O3I 和炎症替代标志物在整个赛季中的影响。
方法
采用多地点、非随机设计，利用两支美式足球队。一队（n=31）在赛前和整个常规赛期间接受高生物利用度 ω-3 FA 配方（2000mg DHA、560mg EPA、320mg DPA、Mindset®、Struct Nutrition、Missoula、MT）补充，而第二队作为对照组（n=35）不接受补充。在强度、身体接触以及头部撞击的发生率和严重程度发生变化时，在赛前和常规赛的特定时间点采集血液样本。通过混合模型的组间和组内方差分析确定组间差异。使用 Cohen's d 计算所有组间差异的效应大小。预先设定显著性水平为 p<.05。
结果
与对照组相比，治疗组的美式足球运动员血浆 EPA（p <.001，d = 1.71）和 DHA（p <.001，d = 2.10）大幅增加，这导致 O3I（p <.001，d = 2.16）和 EPA:AA 比值（p=.001，d = 0.83）增加，而 ω-6:ω-3 比值（p <.001，d = 1.80）降低。ω-3 FA 补充可降低 Nf-L 升高（p=.024）。对照组在几个测量时间点（T2、T3 和 T4 [p 范围 <.001-.005，d 范围 = 0.59-0.85]）与基线相比，Nf-L 显著增加。
结论
这些发现表明，在美式足球运动员中，联合 EPA+DPA+DHA ω-3 FA 补充具有心脏和神经保护作用。
试验注册
本试验在 ISRCTN 注册（ISRCTN90306741）。
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