Sun Jing, Liu Jia-Ni, Li Yue-Ting, Huo Hui-Xia, Xu Xia, Xia Hui, Song Yue-Lin, Zhang Qing-Ying, Tu Peng-Fei, Li Jun
Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China Laboratory of Quality & Safety Risk Assessment on Agro-products Processing of the Ministry of Agriculture and Rural Affairs,Key Laboratory of Agricultural Product Quality and Safety Management and Control of the Ministry of Agriculture and Rural Affairs,Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences Beijing 100193,China.
Laboratory of Quality & Safety Risk Assessment on Agro-products Processing of the Ministry of Agriculture and Rural Affairs,Key Laboratory of Agricultural Product Quality and Safety Management and Control of the Ministry of Agriculture and Rural Affairs,Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences Beijing 100193,China.
Zhongguo Zhong Yao Za Zhi. 2021 Sep;46(18):4841-4848. doi: 10.19540/j.cnki.cjcmm.20210512.201.
Longxue Tongluo Capsules(LTC) has good efficacy against blood stasis syndrome during the recovery period of ischemic stroke. Its main active ingredient is the phenolic extract of Chinese dragon's blood. In our previous study, the primary mass fragmentation pathways of phenolic derivatives from LTC were clarified. Herein, the metabolites in rat plasma were characterized following the oral administration of loureirin A and loureirin C using liquid chromatography coupled with hybrid ion trap/time-of-flight mass spectro-metry(LC-IT-TOF-MS), with 18 and 55 metabolites identified, respectively. On this basis, with the help of the obtained accurate molecular weight, characteristic fragment ions, reference comparison, combined with LTC database and natural products database self-created in our group, 18 prototypes and 106 metabolites were tentatively identified in rat plasma after oral gavage of LTC at a dose of 500 mg·kg~(-1). Glucuronidation, sulfonation, and methylation were major biotransformation pathways of LTC. This study preliminarily clarified the LTC constituents absorbed into blood and laid the foundation for clarifying the effective substances of LTC.
龙血通络胶囊（LTC）对缺血性中风恢复期血瘀证具有良好疗效。其主要活性成分是国产血竭的酚类提取物。在我们之前的研究中，已阐明了LTC中酚类衍生物的主要质谱裂解途径。在此，采用液相色谱-混合离子阱/飞行时间质谱（LC-IT-TOF-MS）对大鼠口服龙血素A和龙血素C后的血浆代谢产物进行了表征，分别鉴定出18种和55种代谢产物。在此基础上，借助所获得的精确分子量、特征碎片离子、参考文献比较，并结合本课题组自建的LTC数据库和天然产物数据库，在大鼠口服500 mg·kg⁻¹剂量的LTC后，在大鼠血浆中初步鉴定出18种原型成分和106种代谢产物。葡萄糖醛酸化、磺酸化和甲基化是LTC的主要生物转化途径。本研究初步阐明了吸收入血的LTC成分，为明确LTC的有效物质奠定了基础。
