Department of Anatomy and Cell Biology, Faculty of Medicine, Saarland University, 66421, Homburg Saar, Germany.
Department of Basic Health Sciences, Faculty for Health Sciences. University Rey Juan Carlos, Av. de Atenas s/n, 28922, Alcorcón, Madrid, Spain.
Adv Exp Med Biol. 2021;1345:85-102. doi: 10.1007/978-3-030-82735-9_8.
This project presents the most important findings of the studies, which we carried out in our laboratory on the decellularization of the rat isolated colonic mucosa. We have also included some details of the experiences gathered with the muscle layer as well as the whole wall of the colon. The question of the cytocompatibility of this new substrate has been addressed with the application of primary cultures of human cells and well-established cell lines. The possible applications in experimental and medical settings will be discussed.
本项目呈现了我们实验室在大鼠离体结肠黏膜去细胞化研究中的最重要发现。我们还包括了一些关于肌肉层和整个结肠壁经验的细节。通过应用原代人细胞培养和成熟的细胞系，解决了这种新基质的细胞相容性问题。还讨论了其在实验和医学环境中的可能应用。
