David H. Koch Institute for Integrative Cancer Research, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States; Department of Anesthesiology, Boston Children's Hospital, Boston, MA, United States; Translational Tissue Engineering Center, Wilmer Eye Institute, Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, United States; Bloomberg∼Kimmel Institute for Cancer Immunotherapy, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Translational Tissue Engineering Center, Wilmer Eye Institute, Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Semin Immunol. 2017 Feb;29:14-23. doi: 10.1016/j.smim.2017.05.002. Epub 2017 Jun 2.
Urinary bladder matrix (UBM) is used clinically for management of wounds and reinforcement of surgical soft tissue repair, among other applications. UBM consists of the lamina propria and basal lamina of the porcine urinary bladder, and is decellularized as part of the process to manufacture the medical device. UBM is composed mainly of Collagen I, but also contains a wide variety of fibrillar and basement membrane collagens, glycoproteins, proteoglycans and ECM-associated factors. Upon application of the biomaterial in a traumatic or non-traumatic setting in a mouse model, there is a cascade of immune cells that respond to the damaged tissue and biomaterial. Here, through the use of multicolor flow cytometry, we describe the various cells that infiltrate the UBM scaffold in a subcutaneous and volumetric muscle injury model. A wide variety of immune cells are found in the UBM scaffold immune microenvironment (SIM) including F4/80 macrophages, CD11c dendritic cells, CD3 T cells and CD19 B cells. A systemic IL-4 upregulation and a local M2-macrophage response were observed in the proximity of the implanted UBM. The recruitment and activation of these cells is dependent upon signals from the scaffold and communication between the different cell types present.
尿囊基质 (UBM) 临床上用于管理伤口和增强手术软组织修复等应用。UBM 由猪膀胱的固有层和基底层组成，在制造医疗器械的过程中被去细胞化。UBM 主要由胶原 I 组成，但也包含各种纤维状和基底膜胶原、糖蛋白、蛋白聚糖和 ECM 相关因子。在创伤或非创伤情况下，将生物材料应用于小鼠模型时，会有一系列免疫细胞对受损组织和生物材料做出反应。在这里，我们通过使用多色流式细胞术，描述了在皮下和容积性肌肉损伤模型中渗透到 UBM 支架中的各种细胞。在 UBM 支架免疫微环境 (SIM) 中发现了各种各样的免疫细胞，包括 F4/80 巨噬细胞、CD11c 树突状细胞、CD3 T 细胞和 CD19 B 细胞。在植入 UBM 的附近观察到全身 IL-4 上调和局部 M2 巨噬细胞反应。这些细胞的募集和激活取决于支架的信号和存在的不同细胞类型之间的通信。
