生殖组织脱细胞化。

Decellularization of Male Reproductive Tissue.

机构信息

In Vitro Toxicology and Dermato-Cosmetology (IVTD), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium.

Biology of the Testis (BITE) Research Group, Department of Reproduction, Genetics and Regenerative Medicine, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium.

出版信息

Adv Exp Med Biol. 2021;1345:161-164. doi: 10.1007/978-3-030-82735-9_14.

DOI:10.1007/978-3-030-82735-9_14

PMID:34582022

Abstract

Decellularized testicular matrix (DTM) enables researchers to focus on the specific composition of the testicular extracellular matrix (ECM) and elucidate its role in spermatogenesis. Furthermore, it provides the natural architectural arrangement that could guide the reorganization of dissociated testicular cells in vitro. This is a key consideration as the presence of an authentic nutritive and endocrine support has been proven to be essential for in vitro spermatogenesis, at least in the mouse (Oliver and Stukenborg in Andrology 8:825-834, 2020; Richer et al. in Andrology 12741, 2019). Hence, scaffolds of DTM could be harnessed for the development of a human in vitro spermatogenesis culture system, which is a missing link in male fertility preservation and could be a possible treatment for nonobstructive azoospermia (Gassei and Orwig in Steril 105:256-266, 2016).

摘要

去细胞化睾丸基质 (DTM) 使研究人员能够专注于睾丸细胞外基质 (ECM) 的特定组成，并阐明其在精子发生中的作用。此外，它提供了自然的结构排列，可以指导分离的睾丸细胞在体外的重新组织。这是一个关键的考虑因素，因为已经证明，存在真实的营养和内分泌支持对于体外精子发生是必不可少的，至少在小鼠中是如此 (Oliver 和 Stukenborg 在《Andrology》8:825-834, 2020 年;Richer 等人在《Andrology》12741, 2019 年)。因此，DTM 的支架可以用于开发人类体外精子发生培养系统，这是男性生育力保存中的一个缺失环节，也可能是治疗非梗阻性无精子症的一种方法 (Gassei 和 Orwig 在《Steril》105:256-266, 2016 年)。