Baert Yoni, Goossens Ellen
Biology of the Testis, Research Laboratory for Reproduction, Genetics and Regenerative Medicine, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium.
Methods Mol Biol. 2018;1577:121-127. doi: 10.1007/7651_2017_29.
Biological scaffolds composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Here we describe a simple and robust methodology for the preparation of decellularized testicular matrix (DTM) scaffolds with minimal damage to the native three-dimensional structure and tissue-specific ECM components. Such DTM scaffolds can help to gain a better insight into the molecular composition and function of testicular ECM and to develop new tissue engineering approaches to treat various types of male fertility disorders.
由细胞外基质（ECM）组成的生物支架通常通过涉及组织或器官去细胞化的过程获得。在此，我们描述了一种简单且可靠的方法，用于制备对天然三维结构和组织特异性ECM成分损伤最小的去细胞化睾丸基质（DTM）支架。这种DTM支架有助于更深入地了解睾丸ECM的分子组成和功能，并开发新的组织工程方法来治疗各种类型的男性生育障碍。
