Srivastava Neha, Mohammad Akbar, Srivastava Manish, Syed Asad, Elgorban Abdallah M, Bahadur Pal Dan, Mishra P K, Yoon Taeho, Gupta Vijai Kumar
Department of Chemical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India.
School of Chemical Engineering, Yeungnam University, Gyeongsan-si, Gyeongbuk 38541, South Korea.
Bioresour Technol. 2021 Dec;342:126006. doi: 10.1016/j.biortech.2021.126006. Epub 2021 Sep 22.
In biomass to biofuels production technology enzyme plays a key role. Nevertheless, the high production cost of cellulase enzyme is one of the critical issues in the economical production of biofuels. Nowadays, implementation of nanomaterials as catalyst is emerging as an innovative approach for the production of sustainable energy. In this context, synthesis of nickel cobaltite nanoparticles (NiCoO NPs) via in vitro route has been conducted using fungus Emericella variecolor NS3 meanwhile; its impact has been evaluated on improved thermal and pH stability of crude cellulase enzyme obtained from Emericella variecolor NS3. Additionally, bioconversion of alkali treated rice straw using NiCoO NPs stabilized cellulase produced sugar hydrolyzate which is further used for H production via hybrid fermentation. Total 51.7 g/L sugar hydrolyzate produced 2978 mL/L cumulative H production after 336 h along with maximum rate 34.12 mL/L/h in 24 h using Bacillus subtilis PF_1 and Rhodobacter sp. employed for dark and photo-fermentation, respectively.
在生物质转化为生物燃料的生产技术中，酶起着关键作用。然而，纤维素酶的高生产成本是生物燃料经济生产中的关键问题之一。如今，将纳米材料用作催化剂正成为一种生产可持续能源的创新方法。在此背景下，已利用真菌变色蜡蚧菌NS3通过体外途径合成了镍钴矿纳米颗粒（NiCoO NPs）；同时，评估了其对提高从变色蜡蚧菌NS3获得的粗纤维素酶的热稳定性和pH稳定性的影响。此外，使用NiCoO NPs稳定的纤维素酶对碱处理稻草进行生物转化产生了糖水解产物，该产物进一步通过混合发酵用于制氢。在336小时后，总共51.7 g/L的糖水解产物使用分别用于暗发酵和光发酵的枯草芽孢杆菌PF_1和红假单胞菌产生了2978 mL/L的累积产氢量，24小时内的最大产氢速率为34.12 mL/L/h。
