Department of General and Environmental Microbiology, Poznań University of Life Sciences, Poznań, Poland.
Pol J Microbiol. 2021 Sep;70(3):339-343. doi: 10.33073/pjm-2021-032. Epub 2021 Sep 17.
The present study aimed to evaluate the effectiveness of low-frequency ultrasounds applied to eliminate spp. from water. The strains used in this research were isolated from water contaminated with sewage. alone was detected in the samples and used for further research. The reference strain ATCC 33559 was simultaneously tested. The isolate was exposed to ultrasounds at frequencies of 37 kHz and 80 kHz in a continuous operation device with ultrapure deionized water. After 5 min of sonication, the count of decreased by 5.78% (37 kHz) and 6.27% (80 kHz), whereas the temperature increased by 3°C (37 kHz), and 6°C (80 kHz). After 30 min of sonication, the death rates of bacterial cells were 40.15% (37 kHz) and 55.10% (80 kHz), whereas the temperature reached the maximum values of 36°C (37 kHz), and 39°C (80 kHz). Sonication at the frequency of 80 kHz reduced the bacterial count from 6.86 log CFU/ml to 3.08 log CFU/ml, whereas the frequency of 37 kHz reduced the bacterial count from 6.75 log CFU/ml to 4.04 log CFU/ml. Despite significant differences ( < 0.05) in the number of cells, the cell death rate remained at the same level.
本研究旨在评估低频超声在消除水中 spp. 方面的效果。本研究中使用的菌株是从受污水污染的水中分离出来的。仅在样本中检测到 ，并用于进一步研究。同时测试了参考菌株 ATCC 33559。将分离株暴露于 37 kHz 和 80 kHz 的连续操作装置中的超纯去离子水中。超声 5 分钟后，37 kHz 时 的数量减少了 5.78%，80 kHz 时减少了 6.27%，而温度分别升高了 3°C 和 6°C。超声 30 分钟后，细菌细胞的死亡率分别为 40.15%（37 kHz）和 55.10%（80 kHz），而温度分别达到了 36°C（37 kHz）和 39°C（80 kHz）的最大值。80 kHz 的超声频率将细菌数量从 6.86 log CFU/ml 降低到 3.08 log CFU/ml，而 37 kHz 的超声频率将细菌数量从 6.75 log CFU/ml 降低到 4.04 log CFU/ml。尽管细胞数量存在显著差异（ < 0.05），但细胞死亡率仍保持在同一水平。
