使用 MULTILAYER 从数字化空间转录组中揭示分子组织亚结构的方案。

Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, University of Evry, University Paris-Saclay, 91057 Évry, France.

STAR Protoc. 2021 Sep 14;2(4):100823. doi: 10.1016/j.xpro.2021.100823. eCollection 2021 Dec 17.

Spatially resolved transcriptomics (SrT) allow researchers to explore organ/tissue architecture from the angle of the gene programs involved in their molecular complexity. Here, we describe the use of MULTILAYER to reveal molecular tissue substructures from the analysis of localized transcriptomes (defined as gexels). MULTILAYER can process low- and high-resolution SrT data but also perform comparative analyses within multiple SrT readouts. For complete details on the use and execution of this protocol, please refer to Moehlin et al., 2021.

空间分辨转录组学（SrT）使研究人员能够从参与其分子复杂性的基因程序的角度探索器官/组织结构。在这里，我们描述了使用 MULTILAYER 从局部转录组（定义为 gexels）的分析中揭示分子组织亚结构。MULTILAYER 可以处理低分辨率和高分辨率 SrT 数据，还可以在多个 SrT 读数内执行比较分析。有关此协议的使用和执行的完整详细信息，请参见 Moehlin 等人，2021 年。