School of Life Science and Technology, the Key Laboratory of Developmental Genes and Human Disease, Southeast University, 2 Sipailou Road, Nanjing 210096, China.
STAR Protoc. 2021 Sep 17;2(4):100827. doi: 10.1016/j.xpro.2021.100827. eCollection 2021 Dec 17.
Sleep behavior is characterized by long-term quiescence and increased arousal threshold, and it is homeostatically regulated. The sleep rebound after deprivation is utilized to verify the abilities to maintain homeostasis. This protocol shows how to build a programmed mechanic oscillation system and detailed procedures to conduct sleep deprivation in a. This deprivation system is featured by its programming flexibility. The knowledge of electronic circuits and a certain level of programming are both required to fulfill this protocol. For complete details on the use and execution of this protocol, please refer to Jin et al. (2021).
睡眠行为的特征是长期的静止和唤醒阈值的增加，并受体内平衡调节。剥夺后的睡眠反弹被用来验证维持体内平衡的能力。本方案展示了如何构建一个程控机械振荡系统，并详细介绍了在 上进行睡眠剥夺的程序。该剥夺系统的特点是编程灵活。要完成本方案，需要具备电子电路知识和一定的编程水平。有关使用和执行本方案的完整详细信息，请参考 Jin 等人（2021 年）。
