Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel.
STAR Protoc. 2021 Sep 16;2(4):100828. doi: 10.1016/j.xpro.2021.100828. eCollection 2021 Dec 17.
Short tandem repeats (STRs) are highly abundant in the human genome, but existing approaches for accurate genotyping of STRs are limited. Here, we describe a protocol for duplex molecular inversion probes for high-throughput and cost-effective STR enrichment. We have successfully tested panels targeting as many as 50K STRs in several thousands of genomic samples (e.g., HeLa cells, Du145 cells, leukemia cells, melanoma cells). However, because the protocol is plate based, the sample size is limited to a few thousand. For complete details on the use and execution of this protocol, please refer to Tao et al. (2021).
短串联重复序列（STRs）在人类基因组中高度丰富，但现有的 STR 精确基因分型方法有限。在这里，我们描述了一种用于高通量和具有成本效益的 STR 富集的双分子反转探针的方案。我们已经成功地在数千个基因组样本（例如 HeLa 细胞、Du145 细胞、白血病细胞、黑色素瘤细胞）中测试了多达 50K STRs 的面板。然而，由于该方案基于平板，因此样本量限制在几千个。有关此方案使用和执行的完整详细信息，请参阅 Tao 等人（2021 年）。
