通过高级流式细胞术和 CITE-seq 分析获取用于蛋白质和基因表达谱分析的小鼠脾髓细胞。
Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq.
机构信息
Center for Infectious Medicine, Department of Medicine, Karolinska Institute, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden.
The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
出版信息
STAR Protoc. 2021 Sep 17;2(4):100842. doi: 10.1016/j.xpro.2021.100842. eCollection 2021 Dec 17.
Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species. For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021).
在这里，我们通过髓系细胞的高级流式细胞术以及通过 CITE-seq 进行单细胞转录组分析和整合的细胞表面蛋白表达，概述了分离和分析小鼠脾树突状细胞 (DC) 的详细方案。该方案为不同组织和物种提供了通用的可转移路线图。有关该方案使用和执行的完整详细信息，请参阅 Lukowski 等人。(2021)。
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