Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Agricultural University, Palampur, 176 062, India.
Department of Veterinary Medicine, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Agricultural University, Palampur, 176 062, India.
Vet Res Commun. 2022 Feb;46(1):101-107. doi: 10.1007/s11259-021-09833-z. Epub 2021 Sep 28.
An outbreak of sheeppox was investigated in a cluster of villages situated in Western Himalayan ranges of a Northern Indian state. Non-migratory sheep (n = 80) of native breeds namely Gaddi and Rampur Bushair were infected and 15 have died. The outbreak started after a few animals contracted the disease during the summer grazing period at the highland pastures from migrating flocks of sheep. This initial outbreak resulted in a further spreading of the disease into the valley. Clinical examination revealed varying degree of cutaneous papular lesions and respiratory distress. Upon necropsy, visceral lesions in the lungs, trachea and kidneys were also found. Clinical and morbid samples were found positive for sheeppox virus using group specific P32 gene and I3L gene based multiplex PCR differentiating sheeppox and goatpox viruses. Histopathological, hematological and blood biochemical analysis also supported the pathology of an acute viral infection. The causative sheeppox virus strain was isolated using lamb testicular cell culture and phylogenetic analysis, based upon P32 and RPO30 genes, showed its clustering with other Indian strains reported from neighboring states. This study demonstrated the spread of sheeppox virus to new niches by migratory sheep flocks leading to establishment of endemic infections in many new pockets of higher Western Himalayas.
在印度北部一个邦的喜马拉雅山西部山区的一个村庄群中，爆发了绵羊痘疫情。非迁徙绵羊（n=80）感染了本地品种的 Gaddi 和 Rampur Bushair，其中 15 只死亡。疫情是在夏季高海拔牧场从迁徙的羊群中感染少数动物后开始的。最初的疫情导致疾病进一步蔓延到山谷。临床检查显示出不同程度的皮肤丘疹病变和呼吸窘迫。尸检时，肺部、气管和肾脏也发现了内脏病变。使用基于群特异性 P32 基因和 I3L 基因的多重 PCR 对临床和病态样本进行检测，发现绵羊痘病毒呈阳性，可区分绵羊痘和山羊痘病毒。组织病理学、血液学和血液生化学分析也支持急性病毒感染的病理学。使用羔羊睾丸细胞培养分离出了致病的绵羊痘病毒株，基于 P32 和 RPO30 基因的系统发育分析表明，它与来自邻近邦的其他印度株聚类。这项研究表明，迁徙的羊群将绵羊痘病毒传播到新的生态位，导致在喜马拉雅山西部的许多新地区建立了地方性感染。
