Microfluidic Ratiometric Photoelectrochemical Biosensor Using a Magnetic Field on a Photochromic Composite Platform: A Proof-of-Concept Study for Magnetic-Photoelectrochemical Bioanalysis.

Integrating a microfluidic sensor with a ratiometric photoelectrochemical (PEC) strategy to build a bioanalysis device for actual sample testing is often limited to large-volume space-resolution equipment and wavelength-dependent or potential-dependent paired photoactive materials. This work reports a microfluidic ratiometric magnetic-photoelectrochemical (M-PEC) biosensor on the photochromic composite platform to solve the above problems. In particular, as a proof-of-concept study, the platform BiWO/amorphous BiOCl nanosheets/BiS (p-BWO-s) mediated by photochromic color centers and the magnetic photoactive secondary antibody marker ZnFeO@AgO are integrated on the microfluidic biosensor. By enhancement of the photochromic color centers, p-BWO-s outputs a considerable photocurrent signal. Meanwhile, the photoactivity of the secondary antibody marker can be changed with a magnetic field; thus, different photocurrent signals can be obtained to realize ratiometric detection. The quenching photocurrent signal without the magnetic field and the difference photocurrent signal under the magnetic field are quantitatively related to the target concentration, which unfolds a novel general strategy for bioanalysis. Different from traditional ratiometric PEC biosensors, this work characterizes the first ratiometric PEC biosensor based on an external magnetic field. Generally speaking, combined with different biorecognition cases, this scheme with good expansibility brings a unique new perspective.