Universidade Federal da Bahia, Instituto de Geociências, Departamento de Oceanografia, Av. Adhemar de Barros, s/n, Ondina, 40170-110 Salvador, BA, Brazil.
An Acad Bras Cienc. 2021 Sep 24;93(suppl 3):e20210296. doi: 10.1590/0001-3765202120210296. eCollection 2021.
Fungi are excellent producers of extracellular enzymes. Therefore, the present study aimed to investigate the screening of marine fungi, which are laccase and manganese peroxidase potential producers, in solid fermentation for future applications in bioremediation processes of contaminated sites. For this purpose, two-level factorial planning was adopted, using time (6 and 15 days) and the absence or presence of oil (0 and 1%) as factors. The semi-quantitative evaluation was carried out by calculating radial growth, enzyme activity and enzyme index by measuring phenol red or syringaldazine oxidation halo. The results showed that all the studied strains showed a positive result for manganese peroxidase production, with an enzymatic activity in solid medium less than 0.61, indicating a strongly positive activity. Through the enzyme index, the study also showed prominence for Penicillium sp. strains, with values > 2. The enzyme index increase in oil presence and the inexpressive use of the genera studied for ligninolytic enzymes production from crude oil demonstrated these data importance for fermentative processes optimization. Considering the ability of these strains to develop into recalcitrant compounds and the potential for manganese peroxidase production, they are indicated for exploitation in various bioremediation technologies, as well as other biotechnological applications.
真菌是细胞外酶的优秀生产者。因此，本研究旨在筛选产漆酶和锰过氧化物酶的海洋真菌，为未来受污染场地的生物修复过程应用提供参考。为此，采用两水平因子设计，以时间（6 天和 15 天）和是否存在油（0%和 1%）为因子。通过测量苯酚红或愈创木酚紫氧化晕来计算径向生长、酶活性和酶指数，进行半定量评价。结果表明，所有研究菌株对锰过氧化物酶的产生均呈阳性，固体培养基中的酶活性小于 0.61，表明活性很强。通过酶指数，研究还表明青霉属菌株的突出性，其值大于 2。在存在油的情况下，酶指数增加，而所研究的属在产生木质素酶方面的表达不佳，这表明这些数据对于发酵过程的优化很重要。考虑到这些菌株将难降解化合物转化为易降解化合物的能力以及产锰过氧化物酶的潜力，它们可用于各种生物修复技术以及其他生物技术应用。
