Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.
Yunwen Hou, Feifei Xue and Yu Fu contribute equally to this work.
Cell Transplant. 2021 Jan-Dec;30:9636897211045970. doi: 10.1177/09636897211045970.
This study aimed to explore the function of CLPTM1L in oral squamous cell carcinoma and mechanism of tumorigenesis. The expression of CLPTM1L was detected by immunohistochemistry. The localization in cells was detected by immunofluorescence. Cell invasion, proliferation, and migration were detected by transwell, CCK-8 and scratch-wound test. The possible characteristics of CLPTM1L were analysed in TCGA, GO, KEGG and String databases. IHC revealed that the expression of CLPTM1L in 92 cases of OSCC tissues was significantly higher ( < 0.01) than 29 cases of normal oral epithelium tissues. The expression of CLPTM1L was significantly higher in oral squamous cell carcinoma in TCGA database. CLPTM1L expression was not significantly correlated with the patients' clinical parameters. High expression of CLPTM1L was associated with worse prognosis. Cox regression analysis demonstrated that the CLPTM1L expression was the significant risk factor. CLPTM1L was mainly localized in the perinuclear cytoplasm. The vitro studies revealed that the knockdown of suppressed invasion, proliferation and migration. CLPTM1L related genes were enriched in protein processing in the endoplasmic reticulum, protein folding, endoplasmic reticulum formation, N-glycan biosynthesis, and protein hydroxylation. Highly expressed CLPTM1L may contribute to a poor prognosis and increase invasion, proliferation and migration of oral squamous cell carcinoma. may play an important role in tumorgenesis and would be a valuable target gene for the treatment of oral squamous cell carcinoma.
本研究旨在探讨 CLPTM1L 在口腔鳞状细胞癌中的功能和肿瘤发生的机制。通过免疫组织化学检测 CLPTM1L 的表达。通过免疫荧光检测细胞内定位。通过 Transwell、CCK-8 和划痕试验检测细胞侵袭、增殖和迁移。在 TCGA、GO、KEGG 和 String 数据库中分析 CLPTM1L 的可能特征。免疫组化显示，92 例口腔鳞状细胞癌组织中 CLPTM1L 的表达明显高于 29 例正常口腔上皮组织（<0.01）。TCGA 数据库中口腔鳞状细胞癌中 CLPTM1L 的表达明显升高。CLPTM1L 的表达与患者的临床参数无显著相关性。CLPTM1L 高表达与预后不良相关。Cox 回归分析表明 CLPTM1L 表达是显著的危险因素。CLPTM1L 主要定位于核周细胞质。体外研究表明，下调 抑制侵袭、增殖和迁移。CLPTM1L 相关基因富集于内质网蛋白质加工、蛋白质折叠、内质网形成、N-糖基化生物合成和蛋白质羟化。高表达的 CLPTM1L 可能导致预后不良，并增加口腔鳞状细胞癌的侵袭、增殖和迁移。 可能在肿瘤发生中起重要作用，将成为治疗口腔鳞状细胞癌的有价值的靶基因。
