Chuncharunee Aporn, Khosuk Poonyawee, Naovarat Rajitpan, Kaliyadan Feroze, Sreekanth Gopinathan Pillai
Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Department of Dermatology, King Faisal University, Kingdom of Saudi Arabia.
Saudi J Biol Sci. 2021 Oct;28(10):5937-5946. doi: 10.1016/j.sjbs.2021.06.056. Epub 2021 Jun 24.
Roxb., family Zingiberaceae, exhibits diverse biological activities. This study was aimed to investigate the anti-inflammatory potential of a major phenolic diarylheptanoid isolated from , ASPP 092 [(3S)-1-(3,4-dihydroxy-phenyl)-7-phenyl-(6E)-6-hepten-3-ol] in an experimentally-induced inflammatory ear edema model in mice. Ear edema in the mice was induced by the topical application of irritant, ethyl phenylpropiolate (EPP). The topical application of ASPP 092 at the edema site was directed immediately after the EPP application. The edematous responses were assessed at different time points by measuring the thickness of each ear before and after the EPP application followed by histopathology analysis. The expressions of major inflammatory cytokines were analyzed by real-time RT-PCR followed by the immunohistochemistry analysis of cyclooxygenase (COX-2). The topical application of ASPP 092 effectively suppressed the EPP-induced edematous formation in the ear of mice. Histopathological analysis showed substantial improvements in epidermal hyperplasia and inflammatory cell infiltration. ASPP 092 treatment also modulated the expressions of inflammatory cytokines including Tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-1β (IL-1β), and Matrix metalloproteinase-13 (MMP-13). The expressions of cyclooxygenases (COX) including COX-1 and COX-2 were significantly reduced by ASPP 092 treatment. For the first time, our results suggest the efficacy of ASPP 092 to suppress experimentally-induced inflammation in a preclinical model in mice; however, a more detailed evaluation of its mechanism of action is necessary before evaluating its efficacy and safety in randomized trials.
姜科植物[具体植物名称未给出，推测为罗勒姜（Roxb.）]具有多种生物活性。本研究旨在探讨从[具体植物名称未给出，推测为罗勒姜（Roxb.）]中分离出的一种主要酚类二芳基庚烷化合物ASPP 092[（3S）-1-（3,4-二羟基苯基）-7-苯基-（6E）-6-庚烯-3-醇]在小鼠实验性诱导的炎性耳水肿模型中的抗炎潜力。通过局部应用刺激性物质苯丙炔酸乙酯（EPP）诱导小鼠耳水肿。在应用EPP后立即在水肿部位局部应用ASPP 092。通过测量EPP应用前后每只耳朵的厚度并进行组织病理学分析，在不同时间点评估水肿反应。通过实时逆转录聚合酶链反应（RT-PCR）分析主要炎性细胞因子的表达，随后对环氧合酶（COX-2）进行免疫组织化学分析。局部应用ASPP 092可有效抑制EPP诱导的小鼠耳部水肿形成。组织病理学分析显示表皮增生和炎性细胞浸润有显著改善。ASPP 092治疗还调节了炎性细胞因子的表达，包括肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、白细胞介素-1β（IL-1β）和基质金属蛋白酶-13（MMP-13）。ASPP 092治疗可显著降低包括COX-1和COX-2在内的环氧合酶（COX）的表达。我们的结果首次表明ASPP 092在小鼠临床前模型中抑制实验性诱导炎症的有效性；然而，在随机试验中评估其疗效和安全性之前，有必要对其作用机制进行更详细的评估。
