Pan I C, Shimizu M, Hess W R
Am J Vet Res. 1978 Mar;39(3):491-7.
A microplaque assay for Vero cell-adapted Lisborn '60 strain of African swine fever virus (L'60-uncloned) and a large plaque-forming strain cloned from the L'60-uncloned strain was developed by an immunoperoxidase method. The immunoperoxidase method can be used to stain microplaques of 3 days after inoculation, whereas the conventional plaque assay requires 5 to 7 days to develop visible plaques. A linear relationship between viral concentration in the inoculum and plaque numbers was observed. Viral titers obtained by both microplaque assay and conventional plaque assay were comparable, and both methods were reproducible and reliable. The viral titer obtained by either one of the plaque assay methods was approximately 0.9 log10 lower than that obtained by the hemadsorption test.
通过免疫过氧化物酶法开发了一种针对适应Vero细胞的非洲猪瘟病毒利伯恩‘60株（L’60未克隆株）以及从该L‘60未克隆株克隆得到的大噬斑形成株的微量噬斑测定法。免疫过氧化物酶法可用于对接种后3天的微量噬斑进行染色，而传统噬斑测定法需要5至7天才能形成可见噬斑。观察到接种物中的病毒浓度与噬斑数量之间呈线性关系。通过微量噬斑测定法和传统噬斑测定法获得的病毒滴度相当，且两种方法均具有可重复性和可靠性。通过任一噬斑测定法获得的病毒滴度比通过血细胞吸附试验获得的病毒滴度低约0.9 log10。
