Pastor M J, Laviada M D, Sanchez-Vizcaino J M, Escribano J M
Departamento de Sanidad Animal, Instituto Nacional de Investigaciones Agrarias, Madrid, Spain.
Can J Vet Res. 1989 Jan;53(1):105-7.
An immunoblotting assay has been adapted to detect antibodies against African swine fever virus. The electrophoretic transfer of proteins and the immunoreaction conditions were optimized, using 4 mA/cm2 of current intensity and 10 micrograms of soluble cytoplasmic antigen of infected cells per strip. Filters of polyvinylidene difluoride showed the highest capacity for protein absorption, but nitrocellulose filters showed lower backgrounds. The specificity and the pattern of the proteins induced by African swine fever virus that react with the antisera were determined in immunoblotting assay, IP30 being the most reactive protein.
一种免疫印迹测定法已被适配用于检测抗非洲猪瘟病毒的抗体。优化了蛋白质的电泳转移和免疫反应条件，使用每厘米2 4毫安的电流强度以及每条带10微克感染细胞的可溶性细胞质抗原。聚偏二氟乙烯滤膜显示出最高的蛋白质吸附能力，但硝酸纤维素滤膜背景较低。在免疫印迹测定法中确定了与抗血清发生反应的非洲猪瘟病毒诱导的蛋白质的特异性和模式，IP30是反应性最强的蛋白质。
