Astuti Rika Indri, Prastya Muhammad Eka, Batubara Irmanida, Budiarti Eka, Ilmiyawati Aulia
Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, IPB Dramaga Campus, Bogor, West Java 16680, Indonesia.
Tropical Biopharmaca Research Center, IPB University, Jl. Taman Kencana No. 3, Bogor, West Java 16128, Indonesia.
Adv Pharmacol Pharm Sci. 2021 Sep 18;2021:2119634. doi: 10.1155/2021/2119634. eCollection 2021.
Research on antioxidants has been gaining worldwide attention because of their essential applications for medicinal purposes. In this study, we conducted bioprospecting of six Asteraceae plants as the source of antiaging and antioxidant agents. Water and chloroform fractions from L., (L.f.) Kuntze, (Cav.), Kunth, (L.) Pruski, and L. were collected and assayed for their in vitro antioxidant activities and potential antiaging properties using the yeast as the model organism. Based on the in vitro assay, the water fractions of showed a strong antioxidant activity. Interestingly, all treatment solutions promoted the stress tolerance phenotype of to strong HO-induced oxidative stress conditions. Moreover, compared with the treatments without plant extract/fraction, all extract and fraction treatments, except the chloroform fractions of , promoted yeast cell longevity. Strong induction of mitochondria activity was found following the treatments with the extracts and fractions of , , and and likely mimicked the calorie restriction-induced lifespan. Interestingly, water fractions significantly upregulated the mRNA transcripts of the Pap1-mediated core environmental stress response, namely, ctt1 gene in . These data indicated that the fractions of Asteraceae plants had potential antioxidant and antiaging activities through various cellular modulations. water fraction has been shown to have antioxidant and antiaging activities in , by modulating stress tolerance response, inducing mitochondrial activity, and increasing the ctt1 gene expression. Compounds analysis identified that water fraction contained some primarily compounds including oxyphyllacinol, valine, and sugiol.
由于抗氧化剂在医学领域的重要应用，其研究已受到全球关注。在本研究中，我们对六种菊科植物进行了生物勘探，以寻找抗衰老和抗氧化剂来源。收集了来自[植物名称1]、[植物名称2]、[植物名称3]、[植物名称4]和[植物名称5]的水相和氯仿相提取物，并以酵母作为模式生物，检测其体外抗氧化活性和潜在的抗衰老特性。基于体外试验，[植物名称6]的水相提取物显示出较强的抗氧化活性。有趣的是，所有处理溶液都能促进酵母对强HO诱导的氧化应激条件的耐受表型。此外，与未添加植物提取物/馏分的处理相比，除了[植物名称7]的氯仿相提取物外，所有提取物和馏分处理均能延长酵母细胞寿命。在用[植物名称8]、[植物名称9]和[植物名称10]的提取物和馏分处理后，发现线粒体活性得到强烈诱导，这可能模拟了热量限制诱导的寿命延长。有趣的是，[植物名称6]的水相提取物显著上调了Pap1介导的核心环境应激反应的mRNA转录本，即酵母中的ctt1基因。这些数据表明，菊科植物的馏分通过各种细胞调节具有潜在的抗氧化和抗衰老活性。[植物名称6]的水相提取物已被证明在酵母中具有抗氧化和抗衰老活性，通过调节应激耐受反应、诱导线粒体活性和增加ctt1基因表达。化合物分析确定，[植物名称6]的水相提取物含有一些主要化合物，包括氧叶花椒素、缬氨酸和杉木醇。
