Fauzya Anninda Faiz, Astuti Rika Indri, Mubarik Nisa Rachmania
Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, IPB Dramaga Campus, Bogor, West Java 16680, Indonesia.
Int J Microbiol. 2019 Aug 14;2019:2145378. doi: 10.1155/2019/2145378. eCollection 2019.
Compared to the widely explored antioxidant activity from the clove bud extract, less data are available regarding the potential pharmacological use of clove leaves. Our study aimed to explore the antioxidant activity of clove leaves extract in the cellular level. Thus, we used the yeast as model organisms. Our data indicate that, following extract treatment (100 ppm), the viability of the stationary phase cells of was higher than without extract and that of calorie restriction treatments. 100 ppm extract treatment also increased cell viability against HO-induced oxidative stress. Those data indicate that the extract could promote oxidative stress tolerance response in yeast cells, which occurred either during the stationary phase or due to exogenous exposure. Higher dose of extract (500 ppm) showed opposite effects, as cell viability was lower than that without treatment. Analysis toward the mitochondrial activity revealed that the extract did not induce mitochondrial activity unlike the calorie restriction treatment. Based on our data, clove leaf extract promotes oxidative stress tolerance response in the yeast , independent to that mitochondrial adaptive ROS signaling which commonly occurs in calorie restriction-induced oxidative stress tolerance response.
与对丁香花蕾提取物广泛研究的抗氧化活性相比，关于丁香叶潜在药理用途的数据较少。我们的研究旨在探索丁香叶提取物在细胞水平的抗氧化活性。因此，我们使用酵母作为模式生物。我们的数据表明，在提取物处理（100 ppm）后，静止期细胞的活力高于未处理组和热量限制处理组。100 ppm提取物处理还提高了细胞对过氧化氢诱导的氧化应激的活力。这些数据表明，该提取物可以促进酵母细胞的氧化应激耐受反应，这种反应既可以在静止期发生，也可以由外源暴露引起。更高剂量的提取物（500 ppm）显示出相反的效果，因为细胞活力低于未处理组。对线粒体活性的分析表明，与热量限制处理不同，该提取物不会诱导线粒体活性。基于我们的数据，丁香叶提取物促进酵母的氧化应激耐受反应，独立于热量限制诱导的氧化应激耐受反应中常见的线粒体适应性活性氧信号传导。
