Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA.
Analyst. 2021 Nov 22;146(23):7109-7117. doi: 10.1039/d1an01417j.
We have designed and synthesized a multifunctional dendritic molecular probe that selectively detects Cu ions potentiometric and fluorometric techniques with low detection limits (3.5 μM in potentiometry, 15 nM in fluorometry). The selective and reversible binding of the molecule with the Cu ion was used to make a solid-state microsensor (diameter of 25 μm) by incorporating the molecular probe into the carbon-based membrane as an ionophore for Cu(II). The Cu(II) microelectrode has a broad linear range of 10 μM to 1 mM with a near Nernstian slope of 30 mV/log [] and detection limit of 3.5 μM. The Cu(II) microsensor has a fast response time (1.5 s), and it has a broad working pH range from 3.5 to 6.0. The incorporation of the hydrophobic dendritic moiety makes the ionophore less prone to leaching in an aqueous matrix for potentiometric measurement. The cinnamaldehyde component of the molecule helps detection of Cu ions fluorometrically, as indicated by a change in fluorescence upon selective and reversible binding of the molecular probe to the Cu ions. The strategic design of the molecular probe allows us to detect Cu ions in drinking water by using this novel dendritic fluoroionophore and solid-state Cu - ion-selective microelectrode.
我们设计并合成了一种多功能树枝状分子探针，该探针可通过电位法和荧光法选择性地检测 Cu 离子，检测限低（电位法为 3.5 μM，荧光法为 15 nM）。该分子与 Cu 离子的选择性和可逆结合被用于通过将分子探针作为 Cu(II)的离子载体掺入基于碳的膜中，来制作固态微传感器（直径为 25 μm）。Cu(II)微电极具有 10 μM 至 1 mM 的宽线性范围，其斜率接近 Nernstian（30 mV/log []），检测限为 3.5 μM。Cu(II)微传感器具有快速的响应时间（1.5 s），其工作 pH 范围从 3.5 到 6.0。由于亲脂性树枝状部分的存在，使得该离子载体在水基中进行电位测量时不易浸出。分子探针的肉桂醛部分有助于通过荧光法检测 Cu 离子，这表明分子探针与 Cu 离子选择性和可逆结合后，荧光发生变化。分子探针的策略性设计使我们能够使用这种新型树枝状荧光离子载体和固态 Cu-离子选择性微电极来检测饮用水中的 Cu 离子。
