Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, Departamento de Enfermagem, Botucatu, SP, Brazil.
Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Departamento de Clínica Veterinária, Botucatu, SP, Brazil.
Rev Esc Enferm USP. 2021 Sep 27;55:e20200527. doi: 10.1590/1980-220X-REEUSP-2020-0527. eCollection 2021.
To analyze wound contraction and histomorphometric pattern of lesions in Wistar rats undergoing doxorubicin extravasation.
Sixty adult female rats were used, divided into four groups of fifteen animals: Group 1 (Control, without antidote); Group 2 (Hyaluronidase); Group 3 (Photobiomodulation), and Group 4 (Hyaluronidase + Photobiomodulation). Doxorubicin 1mg (0.5 ml) was applied subcutaneously on the animals' back, inducing the wound. Macroscopic and morphometric evaluation of the lesions was performed every two days for 28 days. On the 30th day, euthanasia was performed and the material was collected for histological evaluation.
The animals in the photobiomodulation and photobiomodulation + Hyaluronidase groups presented regeneration tissue with neovascularization and acute inflammation, with improvement in wound healing, which did not occur in the other groups. The contraction rates were better in those treated with photobiomodulation and photobiomodulation + Hyaluronidase, with healing percentages of 76.6% and 72.1%, respectively.
The combination of photobiomodulation (660 nm-1 J) with topical hyaluronidase (65 UTR) proved to be effective in the process of wound healing due to extravasation of doxorubicin, and can be incorporated into the practice of clinical oncology.
分析多柔比星外渗的 Wistar 大鼠创面收缩和病变组织形态计量学模式。
使用 60 只成年雌性大鼠，分为 4 组，每组 15 只动物：第 1 组（对照组，无解毒剂）；第 2 组（透明质酸酶）；第 3 组（光生物调节）和第 4 组（透明质酸酶+光生物调节）。在动物背部皮下应用 1mg（0.5ml）多柔比星，诱导伤口。在 28 天内每两天对病变进行宏观和形态计量评估。第 30 天进行安乐死，并收集组织进行组织学评估。
光生物调节和光生物调节+透明质酸酶组的动物表现出具有新生血管和急性炎症的再生组织，伤口愈合得到改善，而其他组则没有。经光生物调节和光生物调节+透明质酸酶治疗的收缩率更好，愈合百分比分别为 76.6%和 72.1%。
多柔比星外渗时，660nm-1J 的光生物调节与局部透明质酸酶（65UTR）联合应用于创面愈合过程中有效，可纳入临床肿瘤学实践。
