Departamento de Fisioterapia, Universidade Federal de São Carlos (UFSCar), São Carlos, SP, Brazil.
Departamento de Fisioterapia, Centro Universitário de Votuporanga (UNIFEV), Rua José Abdo Marão, 3439 - Bairro Jardim Marin, Votuporanga, SP, 15501-031, Brazil.
Lasers Med Sci. 2018 Dec;33(9):1867-1874. doi: 10.1007/s10103-018-2535-4. Epub 2018 May 22.
Wound healing is a complex biological process with specific phases. Photobiomodulation (PBM) decreases the inflammatory infiltrate, stimulating fibroblast proliferation and angiogenesis, and therefore, is indicated for wound healing. Vitamin A is used to reverse the inhibitory effects on wound healing and accelerate the healthy granulation tissue. The study aimed to evaluate the effect of topical vitamin A and PBM (GaAlAs) in inflammatory phase of cutaneous wounds. Forty Wistar male rats were separated into four groups: (1) control (CG); (2) laser group (LG) GaAlAs, 670 nm, 30 mW, energy per point of 0.9 J, radiating by 1 point in 30 s; (3) vitamin A group (VitAG); and (4) laser group plus vitamin A (LG + VitAG). Wounds were surgically made by a punch biopsy with 10 mm of diameter on the back of the animals and all treatments were started according to the experiment. The treatments were administered for four consecutive days and biopsy was performed on day 4. We performed both H&E and immunohistochemistry analysis. The results were compared between groups by one-way analysis of variance ANOVA test with post hoc Tukey (p < 0.05). Inflammatory infiltrate increased significantly in LG compared to CG and VitAG (p < 0.05). Regarding angiogenesis, VEGF expression was increased significantly in LG and LG + VitAG groups, p < 0.01. The results indicate that proposed treatments were effective on the healing process improved by LG and LG + VitAG. We show that laser plus vitamin A enhances healing by reducing the wound area and may have potential application for clinical management of cutaneous wounds.
伤口愈合是一个具有特定阶段的复杂生物学过程。光生物调节（PBM）可减少炎症浸润，刺激成纤维细胞增殖和血管生成，因此适用于伤口愈合。维生素 A 用于逆转对伤口愈合的抑制作用并加速健康肉芽组织的形成。本研究旨在评估局部应用维生素 A 和 PBM（GaAlAs）对皮肤伤口炎症期的影响。将 40 只雄性 Wistar 大鼠分为四组：（1）对照组（CG）；（2）激光组（LG）GaAlAs，670nm，30mW，每个点的能量为 0.9J，通过 30s 辐射 1 点；（3）维生素 A 组（VitAG）；和（4）激光组加维生素 A（LG+VitAG）。通过动物背部的 10mm 直径的打孔活检手术制造伤口，并且根据实验开始所有治疗。连续治疗 4 天，在第 4 天进行活检。我们进行了 H&E 和免疫组织化学分析。通过方差分析 ANOVA 测试和事后 Tukey 检验（p<0.05）比较组间结果。与 CG 和 VitAG 相比，LG 中的炎症浸润显著增加（p<0.05）。关于血管生成，LG 和 LG+VitAG 组的 VEGF 表达显著增加（p<0.01）。结果表明，LG 和 LG+VitAG 提出的治疗方法有效改善了愈合过程。我们表明，激光加维生素 A 通过减少伤口面积来增强愈合，并且可能对皮肤伤口的临床管理具有潜在的应用。
