Kogo H, Iida H, Inazu N, Satoh T
Prostaglandins Leukot Med. 1986 Apr;22(1):11-20. doi: 10.1016/0262-1746(86)90018-1.
The present study was designed to investigate the effects of chlorpromazine (CPZ) on the formation of 13,14H2-PGF2 alpha in rat ovary and the possibility that the "critical period" observed in the effect of CPZ for the blockade of ovulatin may be appreciated by measuring the formation of 13,14H2-PGF2 alpha as a parametor. When CPZ (4.0 mg/kg) was given s.c. at 10:00 a.m. once a day for 3 days from the first day of diestrus to the expected day of proestrus followed by sacrifice after 24 hours of the final injection, a significant inhibition was recognized in the formation of 13,14H2-PGF2 alpha in ovary. The effects of a single injection of CPZ were also determined 24 hours after injection at 10:00 a.m. on the day of diestrus I, diestrus II or proestrus. The results showed that the drug treatment on the day of proestrus significantly decreased the formation of 13,14H2-PGF2 alpha, but not on the day of diestrus I or II. When the formation of ovarian 13,14H2-PGF2 alpha was inhibited by CPZ treatment, the blockade of ovulation was also confirmed. A single injection of CPZ at 05:00 and 07:00 after the "critical period" on the day of proestrus did not inhibit the 13,14H2-PGF2 alpha formation in the rat ovary. It is concluded that the administration of CPZ before the "critical period" on the day of proestrus is effective in suppressing the ovarian 13,14H2-PGF2 alpha formation as well as on the blockade of ovulation.
本研究旨在探讨氯丙嗪（CPZ）对大鼠卵巢中13,14H2-PGF2α形成的影响，以及通过测量13,14H2-PGF2α的形成作为参数来评估CPZ阻断排卵作用中观察到的“关键期”的可能性。从间情期第一天至预期的发情前期，每天上午10:00皮下注射CPZ（4.0mg/kg），连续3天，最后一次注射24小时后处死大鼠，结果发现卵巢中13,14H2-PGF2α的形成受到显著抑制。在间情期I、间情期II或发情前期的上午10:00注射CPZ 24小时后，也测定了单次注射CPZ的效果。结果显示，发情前期给药显著降低了13,14H2-PGF2α的形成，但在间情期I或II给药则无此效果。当CPZ处理抑制卵巢13,14H2-PGF2α的形成时，也证实了排卵受到阻断。在发情前期“关键期”之后的05:00和07:00单次注射CPZ，并未抑制大鼠卵巢中13,14H2-PGF2α的形成。结论是，在发情前期的“关键期”之前给予CPZ可有效抑制卵巢13,14H2-PGF2α的形成以及阻断排卵。
