Inazu N, Kogo H, Aizawa Y
Jpn J Pharmacol. 1984 Jun;35(2):87-93. doi: 10.1254/jjp.35.87.
In order to examine a possible role of the pituitary gland and uterine tissue in the formation of 13,14-dihydroprostaglandin F2 alpha (13,14H2-PGF2 alpha) in rat ovary, hypophysectomized and hysterectomized rats were used. Gonadotropins stimulated the formation of 13,14H2-PGF2 alpha from prostaglandin F2 alpha (PGF2 alpha) and 13,14-dihydro-15-keto-PGF2 alpha (15KD-PGF2 alpha) in the ovarian homogenate of hypophysectomized and hysterectomized rats as well as in intact rats. Ovarian steroids, estradiol and progesterone, reduced the formation of 13,14H2-PGF2 alpha in the ovarian homogenate of intact rats. However, in hysterectomized and hypophysectomized rats, its formation was not affected by ovarian steroids. On the other hand, when pregnant mare serum gonadotropin (PMS) and estradiol were simultaneously administered to intact and hypophysectomized rats, the formation of 13,14H2-PGF2 alpha in the ovary showed a tendency to be increased as compared with that after treatment with PMS alone. These results not only suggest that the formation of 13,14H2-PGF2 alpha in rat ovary is regulated by gonadotropins and ovarian steroids, but also that uterine tissue may take part in the process of its formation.
为了研究垂体和子宫组织在大鼠卵巢中13,14-二氢前列腺素F2α（13,14H2-PGF2α）形成过程中可能发挥的作用，研究人员使用了垂体切除和子宫切除的大鼠。促性腺激素可刺激垂体切除和子宫切除大鼠以及完整大鼠的卵巢匀浆中，由前列腺素F2α（PGF2α）和13,14-二氢-15-酮-PGF2α（15KD-PGF2α）生成13,14H2-PGF2α。卵巢类固醇，即雌二醇和孕酮，可减少完整大鼠卵巢匀浆中13,14H2-PGF2α的生成。然而，在子宫切除和垂体切除的大鼠中，其生成不受卵巢类固醇的影响。另一方面，当向完整和垂体切除的大鼠同时注射孕马血清促性腺激素（PMS）和雌二醇时，与单独使用PMS处理后的情况相比，卵巢中13,14H2-PGF2α的生成呈现出增加的趋势。这些结果不仅表明大鼠卵巢中13,14H2-PGF2α的生成受促性腺激素和卵巢类固醇的调节，还表明子宫组织可能参与了其形成过程。
