State Key Laboratory of Southwestern Chinese Medicine Resources, And Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, PR China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, And Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, PR China.
Phytochemistry. 2021 Dec;192:112961. doi: 10.1016/j.phytochem.2021.112961. Epub 2021 Sep 27.
Two undescribed secoiridoids (dehydroxyl-swerimilegenin H and 9-oxo-swerimuslactone A) with eight known ones, and two undescribed triterpenoids [28-O-(3,4-dihydroxyl-benzyl)-lupeol and 17-hydroperoxide-28-norurs-12-en-3-one] with seven known ones, were isolated from the aerial parts of Gentiana veitchiorum, a traditional Tibetan medicine. The structures of these compounds were elucidated by detailed spectroscopic analyses (including 1D and 2D NMR, HRMS, IR, and specific rotation) and comparison with structurally related known compounds. The isolates were selected to evaluate for their immunosuppressive activity via inhibiting the proliferation of T cells and cytokine IFN-γ production in T cells. Among them, 28-O-(3,4-dihydroxyl-benzyl)-lupeol exhibited significant effect by inhibiting the proliferation of T cells (IC, 20.08 μM) and T cell IFN-γ production (IC, 7.29 μM).
从藏药秦艽Gentiana veitchiorum 的地上部分分离得到了两个未知的倍半萜类化合物（去羟基-swerimilegenin H 和 9-氧代-swerimuslactone A）和 8 个已知的倍半萜类化合物，以及两个未知的三萜类化合物[28-O-(3,4-二羟基苄基）-羽扇豆醇和 17-过氧化物-28-降乌苏-12-烯-3-酮]和 7 个已知的三萜类化合物。这些化合物的结构通过详细的光谱分析（包括 1D 和 2D NMR、HRMS、IR 和比旋光度）和与结构相关的已知化合物进行比较来阐明。选择这些化合物来评估它们的免疫抑制活性，通过抑制 T 细胞的增殖和 T 细胞中细胞因子 IFN-γ的产生。其中，28-O-(3,4-二羟基苄基）-羽扇豆醇通过抑制 T 细胞的增殖（IC，20.08 μM）和 T 细胞 IFN-γ的产生（IC，7.29 μM）表现出显著的效果。
