State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.
Ecotoxicol Environ Saf. 2021 Dec 15;226:112809. doi: 10.1016/j.ecoenv.2021.112809. Epub 2021 Sep 27.
Dinotefuran is a widely used neonicotinoid pesticides in agriculture and it has certain ecological toxicity to aquatic organisms. Studies on the potential toxicological effects of dinotefuran on fish are limited. In the present study, 96 h acute toxicity test indicated that enantiomers of R-(-)-dinotefuran had a greater toxic effect than Rac-dinotefuran on zebrafish, and S-(+)-dinotefuran was the least. In chronic assay, R-(-)-dinotefuran exerted more effects on the development of zebrafish than S-(+)-dinotefuran, and dinotefuran also had enantioselective effect on oxidative stress. Significant changes were observed in the superoxide dismutase (SOD), glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities and malondialdehyde (MDA) contents, which demonstrated dinotefuran induced oxidative stress in zebrafish. Besides, through an ultra-performance liquid chromatography quadrupole-TOF mass spectrometry (UPLC-Q-TOF-MS)-based metabolomics method was used to evaluate the enantioselectivity of dinotefuran enantiomers in zebrafish. The results indicated that R-(-)-dinotefuran caused greater disturbances of endogenous metabolites. Phenylalanine metabolic pathways, glycine, serine and threonine metabolic pathways are only involved in zebrafish exposed to R-(-)-dinotefuran; whereas phenylalanine, tyrosine and tryptophan biosynthesis was only involved in zebrafish exposed to S-(+)-dinotefuran. This study provides a certain reference value for assessing the environmental risks of dinotefuran enantiomers to aquatic organisms, and has practical significance for guiding the ecologically and environmentally safety use of dinotefuran.
呋虫胺是一种广泛应用于农业的新烟碱类杀虫剂，对水生生物具有一定的生态毒性。目前关于呋虫胺对鱼类潜在毒理学效应的研究较少。本研究通过 96 h 急性毒性试验表明，呋虫胺对斑马鱼的毒性，其对映体 R-(-)-呋虫胺大于外消旋体 Rac-呋虫胺，而 S-(+)-呋虫胺的毒性最小。在慢性试验中，R-(-)-呋虫胺对斑马鱼的发育毒性大于 S-(+)-呋虫胺，并且呋虫胺也对氧化应激具有对映选择性。超氧化物歧化酶（SOD）、谷胱甘肽 S-转移酶（GST）和乙酰胆碱酯酶（AChE）活性和丙二醛（MDA）含量发生显著变化，表明呋虫胺诱导了斑马鱼的氧化应激。此外，通过超高效液相色谱四极杆飞行时间质谱（UPLC-Q-TOF-MS）-基于代谢组学的方法来评估呋虫胺对映体在斑马鱼中的对映选择性。结果表明，R-(-)-呋虫胺对内源性代谢物造成更大的干扰。只有暴露于 R-(-)-呋虫胺的斑马鱼中涉及苯丙氨酸代谢途径、甘氨酸、丝氨酸和苏氨酸代谢途径；而只有暴露于 S-(+)-呋虫胺的斑马鱼中涉及苯丙氨酸、酪氨酸和色氨酸的生物合成。本研究为评估呋虫胺对映体对水生生物的环境风险提供了一定的参考价值，对指导呋虫胺生态和环境安全使用具有实际意义。
