Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, 550025, China.
Environ Sci Pollut Res Int. 2022 Oct;29(47):70833-70841. doi: 10.1007/s11356-022-20424-6. Epub 2022 May 19.
Dinotefuran is a chiral insecticide widely used to control Nilaparvata lugens in agriculture. However, little is known about the toxic effects of dinotefuran enantiomers on aquatic organisms. In this study, zebrafish were exposed to 1.00 and 10.00 mg/L dinotefuran enantiomers for 96 h, after which multivariate pattern recognition, metabolite identification, and pathway analysis were performed. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were then conducted to reveal the metabolic perturbations caused by dinotefuran enantiomers. Metabolic pathway analysis revealed the perturbation of five main pathways, including phenylalanine, tyrosine and tryptophan biosynthesis; phenylalanine metabolism; retinol metabolism; arginine and proline metabolism; and glycerophospholipid metabolism. These disturbed metabolic pathways were strongly correlated with energy, amino acid metabolism, and lipid metabolism. Pathway analysis also indicated that the metabolic pathway changes induced by the same level of R and S-dinotefuran were enantioselective. Our research may provide better insight into the risk of chiral dinotefuran in aquatic organisms in the environment.
呋虫胺是一种手性杀虫剂，广泛用于农业防治褐飞虱。然而，关于呋虫胺对水生生物的毒性作用知之甚少。在这项研究中，斑马鱼在 1.00 和 10.00 mg/L 的呋虫胺对映体中暴露 96 h 后，进行了多元模式识别、代谢物鉴定和途径分析。然后进行主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA），以揭示呋虫胺对映体引起的代谢扰动。代谢途径分析显示，包括苯丙氨酸、酪氨酸和色氨酸生物合成；苯丙氨酸代谢；视黄醇代谢；精氨酸和脯氨酸代谢；和甘油磷脂代谢在内的五条主要途径受到干扰。这些受干扰的代谢途径与能量、氨基酸代谢和脂质代谢密切相关。途径分析还表明，相同水平的 R 和 S-呋虫胺诱导的代谢途径变化具有对映选择性。我们的研究可能为环境中手性呋虫胺对水生生物的风险提供更好的认识。
