使用聚凝胺对CHO细胞进行高频转染。
High-frequency transfection of CHO cells using polybrene.
作者信息
Chaney W G, Howard D R, Pollard J W, Sallustio S, Stanley P
出版信息
Somat Cell Mol Genet. 1986 May;12(3):237-44. doi: 10.1007/BF01570782.
PMID:3459255
Abstract
High-frequency transfection of CHO cells has been achieved for several plasmids, a cosmid library, and genomic DNA using Polybrene and dimethyl sulfoxide. All plasmid transfectants examined were stable and exhibited plasmid sequences in genomic DNA. The method is simple, reproducible, and succeeded with several independent CHO clones in the presence or the absence of carrier DNA, even at very low concentrations of plasmid DNA.
摘要
使用聚凝胺和二甲基亚砜，已实现了对几种质粒、一个黏粒文库和基因组DNA进行CHO细胞的高频转染。所有检测的质粒转染子都是稳定的，并且在基因组DNA中表现出质粒序列。该方法简单、可重复，并且在有或没有载体DNA的情况下，甚至在非常低浓度的质粒DNA条件下，都能在几个独立的CHO克隆中成功实现。
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