多形汉逊酵母中芳香族氨基酸营养缺陷型的选择性富集。
Selective enrichment of aromatic amino acid auxotrophs in Hansenula polymorpha.
作者信息
Sanchez S, Cea A, Flores M E
出版信息
Appl Environ Microbiol. 1978 Feb;35(2):228-30. doi: 10.1128/aem.35.2.228-230.1978.
Abstract
Aromatic amino acid auxotrophs of the methanol-utilizing yeast Hansenula polymorpha were effectively selected by the use of nystatin and a medium that inhibits the growth of tyrosine auxotrophs. The procedure resulted in a frequency of aromatic auxotrophs of 2% of survivors and an enrichment of 20-fold. The new procedure also takes less time than traditional procedures. Of the auxotrophic mutants isolated, two-thirds required tyrosine and the remainder were tyrosine-phenylalanine double auxotrophs.
摘要
通过使用制霉菌素和一种抑制酪氨酸营养缺陷型生长的培养基，有效地筛选出了利用甲醇的多形汉逊酵母的芳香族氨基酸营养缺陷型。该方法使芳香族营养缺陷型在存活者中的频率达到2%，富集了20倍。新方法也比传统方法耗时更少。在分离出的营养缺陷型突变体中，三分之二需要酪氨酸，其余为酪氨酸-苯丙氨酸双重营养缺陷型。
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本文引用的文献
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Screening of auxotrophic mutants of Schizosaccharomyces pombe with 2-deoxyglucose.用2-脱氧葡萄糖筛选粟酒裂殖酵母的营养缺陷型突变体。
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The selection of auxotrophs of Penicillium chrysognystatin.
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