DeGlyPHER：一种分析病毒刺突糖型的超灵敏方法。
DeGlyPHER: An Ultrasensitive Method for the Analysis of Viral Spike -Glycoforms.
机构信息
Department of Molecular Medicine, The Scripps Research Institute, La Jolla, California 92037, United States.
Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, California 92037, United States.
出版信息
Anal Chem. 2021 Oct 12;93(40):13651-13657. doi: 10.1021/acs.analchem.1c03059. Epub 2021 Oct 1.
Abstract
Viruses can evade the host immune system by displaying numerous glycans on their surface "spike-proteins" that cover immune epitopes. We have developed an ultrasensitive "single-pot" method to assess glycan occupancy and the extent of glycan processing from high-mannose to complex forms at each -glycosylation site. Though aimed at characterizing glycosylation of viral spike-proteins as potential vaccines, this method is applicable for the analysis of site-specific glycosylation of any glycoprotein.
摘要
病毒可以通过在其表面的“刺突蛋白”上展示许多聚糖来逃避宿主免疫系统，这些聚糖覆盖了免疫表位。我们开发了一种超灵敏的“单管”方法来评估每个糖基化位点的聚糖占有率和聚糖加工程度，从高甘露糖到复杂形式。虽然该方法旨在表征作为潜在疫苗的病毒刺突蛋白的糖基化，但它也适用于任何糖蛋白的位点特异性糖基化分析。
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