Wolff H H, Scherer R, Maciejewski W, Braun-Falco O
Arch Dermatol Res. 1978 Feb 15;261(1):17-26. doi: 10.1007/BF00455371.
A leukocytoclastic vasculitis was induced by intracutaneous injection of streptococcal antigen in a patient with erythema elevatum diutinum (E.e.d.). The immunoelectronmicroscopical demonstration of C3 was performed by use of the peroxidase-antiperoxidase multistep technique 24 h after the injection of the antigen. Deposits of C3 were found between endothelial cells, on the outer surface of endothelial cells, pericytes, and smooth muscle cells, as well as within the multilayered basal lamina of small vessels. Intact and disintegrating neutrophils accumulate within the vessel walls and in their surroundings. Necrosis and fibrin deposition are present in advanced stages. The findings demonstrate the sequence of events in leukocytoclastic vasculitis at the ultrastructural level. They also support the hypothesis that in E.e.d. an Arthus type reaction induced by bacterial antigens may be of pathogenetic significance.
在一位持久性隆起性红斑（E.e.d.）患者中，通过皮内注射链球菌抗原诱发了白细胞破碎性血管炎。在注射抗原24小时后，使用过氧化物酶-抗过氧化物酶多步技术进行了C3的免疫电子显微镜检测。发现C3沉积在内皮细胞之间、内皮细胞外表面、周细胞和平滑肌细胞上，以及小血管的多层基膜内。完整的和正在解体的中性粒细胞积聚在血管壁及其周围。晚期出现坏死和纤维蛋白沉积。这些发现展示了白细胞破碎性血管炎在超微结构水平上的一系列事件。它们也支持了这样的假说，即在E.e.d.中，由细菌抗原诱导的Arthus型反应可能具有致病意义。
