OsFtsH2 在水稻叶绿体发育中的重要作用。

The essential roles of OsFtsH2 in developing the chloroplast of rice.

机构信息

School of Biological and Chemical Engineering, NingboTech University, Ningbo, 315100, China.

Ningbo Research Institute, Zhejiang University, Ningbo, 315100, China.

出版信息

BMC Plant Biol. 2021 Oct 1;21(1):445. doi: 10.1186/s12870-021-03222-z.

BACKGROUND

Filamentation temperature-sensitive H (FtsH) is an ATP-dependent zinc metalloprotease with ATPase activity, proteolysis activity and molecular chaperone-like activity. For now, a total of nine FtsH proteins have been encoded in rice, but their functions have not revealed in detail. In order to investigate the molecular mechanism of OsFtsH2 here, several osftsh2 knockout mutants were successfully generated by the CRISPR/Cas9 gene editing technology.

RESULTS

All the mutants exhibited a phenotype of striking albino leaf and could not survive through the stage of three leaves. OsFtsH2 was located in the chloroplast and preferentially expressed in green tissues. In addition, osftsh2 mutants could not form normal chloroplasts and had lost photosynthetic autotrophic capacity. RNA sequencing analysis indicated that many biological processes such as photosynthesis-related pathways and plant hormone signal transduction were significantly affected in osftsh2 mutants.

CONCLUSIONS

Overall, the results suggested OsFtsH2 to be essential for chloroplast development in rice.

背景

Filamentation temperature-sensitive H（FtsH）是一种依赖于 ATP 的锌金属蛋白酶，具有 ATP 酶活性、蛋白水解活性和分子伴侣样活性。目前，水稻中共编码了 9 种 FtsH 蛋白，但它们的功能尚未详细揭示。为了研究 OsFtsH2 的分子机制，本研究利用 CRISPR/Cas9 基因编辑技术成功生成了几个 osftsh2 敲除突变体。