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OsFtsH2 在水稻叶绿体发育中的重要作用。

The essential roles of OsFtsH2 in developing the chloroplast of rice.

机构信息

School of Biological and Chemical Engineering, NingboTech University, Ningbo, 315100, China.

Ningbo Research Institute, Zhejiang University, Ningbo, 315100, China.

出版信息

BMC Plant Biol. 2021 Oct 1;21(1):445. doi: 10.1186/s12870-021-03222-z.

DOI:10.1186/s12870-021-03222-z
PMID:34598671
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8485545/
Abstract

BACKGROUND

Filamentation temperature-sensitive H (FtsH) is an ATP-dependent zinc metalloprotease with ATPase activity, proteolysis activity and molecular chaperone-like activity. For now, a total of nine FtsH proteins have been encoded in rice, but their functions have not revealed in detail. In order to investigate the molecular mechanism of OsFtsH2 here, several osftsh2 knockout mutants were successfully generated by the CRISPR/Cas9 gene editing technology.

RESULTS

All the mutants exhibited a phenotype of striking albino leaf and could not survive through the stage of three leaves. OsFtsH2 was located in the chloroplast and preferentially expressed in green tissues. In addition, osftsh2 mutants could not form normal chloroplasts and had lost photosynthetic autotrophic capacity. RNA sequencing analysis indicated that many biological processes such as photosynthesis-related pathways and plant hormone signal transduction were significantly affected in osftsh2 mutants.

CONCLUSIONS

Overall, the results suggested OsFtsH2 to be essential for chloroplast development in rice.

摘要

背景

Filamentation temperature-sensitive H（FtsH）是一种依赖于 ATP 的锌金属蛋白酶，具有 ATP 酶活性、蛋白水解活性和分子伴侣样活性。目前，水稻中共编码了 9 种 FtsH 蛋白，但它们的功能尚未详细揭示。为了研究 OsFtsH2 的分子机制，本研究利用 CRISPR/Cas9 基因编辑技术成功生成了几个 osftsh2 敲除突变体。

结果

所有突变体均表现出明显白化叶的表型，无法通过三叶期。OsFtsH2 定位于叶绿体，在绿色组织中优先表达。此外，osftsh2 突变体不能形成正常的叶绿体，丧失了光合作用的自养能力。RNA 测序分析表明，osftsh2 突变体中许多生物过程，如光合作用相关途径和植物激素信号转导，均受到显著影响。

结论

总体而言，研究结果表明 OsFtsH2 对水稻叶绿体发育至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/04cbdcae693e/12870_2021_3222_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/46f5b3c13198/12870_2021_3222_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/9c5604f00760/12870_2021_3222_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/b4ec6c47730a/12870_2021_3222_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/cee70add6027/12870_2021_3222_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/701fff44b770/12870_2021_3222_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/3ad576c5ef28/12870_2021_3222_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/ec19d05b5683/12870_2021_3222_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/475e850270b9/12870_2021_3222_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/7e91f90b68eb/12870_2021_3222_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/04cbdcae693e/12870_2021_3222_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/46f5b3c13198/12870_2021_3222_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/9c5604f00760/12870_2021_3222_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/b4ec6c47730a/12870_2021_3222_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/cee70add6027/12870_2021_3222_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/701fff44b770/12870_2021_3222_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/3ad576c5ef28/12870_2021_3222_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/ec19d05b5683/12870_2021_3222_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/475e850270b9/12870_2021_3222_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/7e91f90b68eb/12870_2021_3222_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aafa/8485545/04cbdcae693e/12870_2021_3222_Fig10_HTML.jpg

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Plant Physiol Biochem. 2020 Nov;156:407-419. doi: 10.1016/j.plaphy.2020.09.025. Epub 2020 Sep 25.
3
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4
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5
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6
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