Guizhou Province, College of Tobacco Science of Guizhou University/ Guizhou Key Laboratory for Tobacco Quality, Huaxi District, Guiyang City, 550025, People's Republic of China.
BMC Genomics. 2022 Jul 12;23(1):503. doi: 10.1186/s12864-022-08719-x.
The filamentous temperature-sensitive H protease (ftsH) gene family plays an important role in plant growth and development. FtsH proteins belong to the AAA protease family. Studies have shown that it is a key gene for plant chloroplast development and photosynthesis regulation. In addition, the ftsH gene is also involved in plant response to stress. At present, the research and analysis of the ftsH gene family are conducted in microorganisms such as Escherichia coli and Oenococcus and various plants such as Arabidopsis, pear, rice, and corn. However, analysis reports on ftsH genes from tobacco (Nicotiana tabacum L.), an important model plant, are still lacking. Since ftsH genes regulate plant growth and development, it has become necessary to systematically study this gene in an economically important plant like tobacco.
This is the first study to analyze the ftsH gene from Nicotiana tabacum L. K326 (NtftsH). We identified 20 ftsH genes from the whole genome sequence, renamed them according to their chromosomal locations, and divided them into eight subfamilies. These 20 NtftsH genes were unevenly distributed across the 24 chromosomes. We found four pairs of fragment duplications. We further investigated the collinearity between these genes and related genes in five other species. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis identified differential expression patterns of NtftsH in different tissues and under various abiotic stress conditions.
This study provides a comprehensive analysis of the NtftsH gene family. The exon-intron structure and motif composition are highly similar in NtftsH genes that belong to the same evolutionary tree branch. Homology analysis and phylogenetic comparison of ftsH genes from several different plants provide valuable clues for studying the evolutionary characteristics of NtftsH genes. The NtftsH genes play important roles in plant growth and development, revealed by their expression levels in different tissues as well as under different stress conditions. Gene expression and phylogenetic analyses will provide the basis for the functional analysis of NtftsH genes. These results provide a valuable resource for a better understanding of the biological role of the ftsH genes in the tobacco plant.
丝状温度敏感 H 蛋白酶（ftsH）基因家族在植物生长发育中起着重要作用。FtsH 蛋白属于 AAA 蛋白酶家族。研究表明，它是植物叶绿体发育和光合作用调控的关键基因。此外，ftsH 基因还参与植物对胁迫的响应。目前，对大肠杆菌和酿酒酵母等微生物以及拟南芥、梨、水稻和玉米等各种植物的 ftsH 基因家族的研究和分析已经展开。然而，对于烟草（Nicotiana tabacum L.）等重要模式植物的 ftsH 基因的分析报告仍然缺乏。由于 ftsH 基因调节植物的生长和发育，因此有必要在烟草等经济上重要的植物中系统地研究这个基因。
这是首次对来自烟草（Nicotiana tabacum L.）K326（NtftsH）的 ftsH 基因进行分析。我们从全基因组序列中鉴定了 20 个 ftsH 基因，根据它们在染色体上的位置重新命名，并将它们分为 8 个亚家族。这 20 个 NtftsH 基因在 24 条染色体上不均匀分布。我们发现了四对片段重复。我们进一步研究了这些基因与其他 5 个物种中相关基因之间的共线性。定量实时聚合酶链反应（qRT-PCR）分析确定了 NtftsH 在不同组织和各种非生物胁迫条件下的差异表达模式。
本研究对 NtftsH 基因家族进行了全面分析。属于同一进化枝的 NtftsH 基因的外显子-内含子结构和基序组成高度相似。来自几种不同植物的 ftsH 基因的同源分析和系统发育比较为研究 NtftsH 基因的进化特征提供了有价值的线索。NtftsH 基因在植物生长发育中发挥重要作用，这从它们在不同组织中的表达水平以及在不同胁迫条件下的表达水平可以看出。基因表达和系统发育分析将为 NtftsH 基因的功能分析提供基础。这些结果为更好地理解 ftsH 基因在烟草植物中的生物学作用提供了有价值的资源。
