Chen Kuen-Lin, Yang Zih-Yan, Lin Chin-Wei
Institute of Nanoscience, National Chung Hsing University, 250, Kuo Kuang Rd., Taichung, 402, Taiwan, ROC.
Department of Physics, National Chung Hsing University, Taichung, Taiwan.
J Nanobiotechnology. 2021 Oct 1;19(1):301. doi: 10.1186/s12951-021-01030-z.
In the past decades, different diseases and viruses, such as Ebola, MERS and COVID-19, impacted the human society and caused huge cost in different fields. With the increasing threat from the new or unknown diseases, the demand of rapid and sensitive assay method is more and more urgent.
In this work, we developed a magneto-optical biochip based on the Cotton-Mouton effect of γ-FeO@Au core/shell magnetic nanoparticles. We performed a proof-of-concept experiment for the detection of the spike glycoprotein S of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The assay was achieved by measuring the magneto-optical Cotton-Mouton effect of the biochip. This magneto-optical biochip can not only be used to detect SARS-CoV-2 but also can be easily modified for other diseases assay.
The assay process is simple and the whole testing time takes only 50 min including 3 min for the CM rotation measurement. The detection limit of our method for the spike glycoprotein S of SARS-CoV-2 is estimated as low as 0.27 ng/mL (3.4 pM).
在过去几十年中，不同的疾病和病毒，如埃博拉病毒、中东呼吸综合征冠状病毒和新型冠状病毒肺炎，对人类社会产生了影响，并在不同领域造成了巨大损失。随着来自新出现或未知疾病的威胁不断增加，对快速且灵敏的检测方法的需求越来越迫切。
在这项工作中，我们基于γ-FeO@Au核壳磁性纳米颗粒的科顿-穆顿效应开发了一种磁光生物芯片。我们针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的刺突糖蛋白S的检测进行了概念验证实验。该检测通过测量生物芯片的磁光科顿-穆顿效应来实现。这种磁光生物芯片不仅可用于检测SARS-CoV-2，还可轻松修改用于其他疾病检测。
检测过程简单，整个测试时间仅需50分钟，其中包括3分钟用于科顿-穆顿旋转测量。我们检测SARS-CoV-2刺突糖蛋白S的方法的检测限估计低至0.27 ng/mL（3.4 pM）。
