Baker Alexander N, Richards Sarah-Jane, Guy Collette S, Congdon Thomas R, Hasan Muhammad, Zwetsloot Alexander J, Gallo Angelo, Lewandowski Józef R, Stansfeld Phillip J, Straube Anne, Walker Marc, Chessa Simona, Pergolizzi Giulia, Dedola Simone, Field Robert A, Gibson Matthew I
Department of Chemistry, University of Warwick, Coventry, CV4 7AL, U.K.
School of Life Sciences, University of Warwick, Coventry, CV4 7AL, U.K.
ACS Cent Sci. 2020 Nov 25;6(11):2046-2052. doi: 10.1021/acscentsci.0c00855. Epub 2020 Sep 23.
There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that -acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.
迫切需要了解新型冠状病毒（SARS-COV-2，即COVID-19的病原体）的行为，并开发即时诊断方法。在此，利用糖纳米颗粒平台发现N-乙酰神经氨酸对SARS-COV-2刺突糖蛋白具有亲和力，证明了其聚糖结合功能。对颗粒大小和涂层进行优化后，能够在侧流检测中检测到刺突糖蛋白，并且对SARS-COV-1刺突蛋白具有选择性。使用病毒样颗粒和假型慢病毒模型，在30分钟内展示了基于纸的侧流检测，显示出该系统作为低成本检测平台的潜力。
