Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 04001, Košice, Slovakia.
Department of Microbiology and Immunology, University of the Veterinary Medicine and Pharmacy, Komenského 73, 04181, Košice, Slovakia.
Vet Res Commun. 2021 Dec;45(4):467-473. doi: 10.1007/s11259-021-09841-z. Epub 2021 Oct 2.
The occurence and spread of animal-derived methicillin resistant staphylococci (MRS) worldwide is a current problem, especially due to their increasing incidence in food animals and their products, with possible contamination of food consumers and handlers. Staphylococci isolated from animals (n = 123) were identified with MALDI-TOF mass spectrometry and screened for methicillin/oxacillin/cefoxitin resistance (MR) using the disk diffusion method. Twenty-three phenotypically MRS strains were analysed using PBP2' Latex Agglutination Test Kit to confirm the phenotypic MR and PCR was performed for mecA gene detection; mecA gene positive strains were furtherly confirmed by means of sequencing. The susceptibility of MRS to 11 partially-purified enterocins (Ent) produced by E. faecium, E. durans and E. mundtii strains of animal, feed/food and environmental origin was checked using agar spot tests. Out of 23 MRS, PBP testing confirmed MR in 17 strains. Three Staphylococcus epidermidis and one S. vitulinus were mecA positive. The majority of MRS, including two mecA gene-positive strains S. epidermidis R44/1 and P3/Tr2a, were susceptible to the tested enterocins, mainly to Ent7420, EntA(P)/EK13, Ent412, Ent55 and Ent9296 (in the range 100 - 12,800 AU/mL). The most susceptible strains appeared to be the mecA gene-positive S. epidermidis SE R44/1 and SE P3/Tr2a strains, inhibited by eight enterocins out of 11 tested (100-200 AU/mL). Only four strains (including mecA gene positive S. epidermidis SE P3/Tr1 and S. vitulinus SV K12PL/1) were resistant to the tested antimicrobial substances. These results indicate that the enterocins used offer a promising option for prevention and treatment of bacterial infection caused by MRS in animals.
动物源耐甲氧西林金黄色葡萄球菌（MRS）在全球范围内的出现和传播是一个当前的问题，特别是由于它们在食品动物及其产品中的发病率不断增加，可能会污染食品消费者和处理者。使用 MALDI-TOF 质谱法对从动物中分离出的金黄色葡萄球菌（n=123）进行鉴定，并使用纸片扩散法筛选耐甲氧西林/苯唑西林/头孢西丁（MR）。使用 PBP2' Latex Agglutination Test Kit 对 23 株表型 MRS 菌株进行分析，以确认表型 MR，并进行 mecA 基因检测；mecA 基因阳性菌株进一步通过测序确认。使用琼脂斑点试验检查 MRS 对 11 种由动物、饲料/食物和环境来源的屎肠球菌、耐久肠球菌和蒙氏肠球菌产生的部分纯化肠球菌（Ent）的敏感性。在 23 株 MRS 中，PBP 检测证实 17 株存在 MR。3 株表皮葡萄球菌和 1 株维鲁氏葡萄球菌 mecA 阳性。大多数 MRS，包括 2 株 mecA 基因阳性的表皮葡萄球菌 R44/1 和 P3/Tr2a，对测试的肠球菌敏感，主要对 Ent7420、EntA(P)/EK13、Ent412、Ent55 和 Ent9296（100-12,800 AU/mL）敏感。最敏感的菌株似乎是 mecA 基因阳性的表皮葡萄球菌 SE R44/1 和 SE P3/Tr2a 菌株，被 11 种测试的肠球菌中的 8 种抑制（100-200 AU/mL）。只有 4 株（包括 mecA 基因阳性的表皮葡萄球菌 SE P3/Tr1 和维鲁氏葡萄球菌 SV K12PL/1）对测试的抗菌物质有耐药性。这些结果表明，使用的肠球菌为预防和治疗动物源 MRS 引起的细菌感染提供了一个有前途的选择。
