Centre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Šoltésovej 4-6, Košice, 04001, Slovakia.
University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 04181, Slovakia.
Vet Res Commun. 2024 Jun;48(3):1449-1457. doi: 10.1007/s11259-024-10323-1. Epub 2024 Feb 7.
There is a major problem with the rising occurrence of highly virulent and multiply-resistant strains, including methicillin-resistant Staphylococcus aureus (MRSA), because of their difficult treatment. This study aimed to evaluate the antibacterial and antibiofilm effect of new enterocins (Ent) against potential pathogenic MRSA strains isolated from rabbits. Staphylococci were identified with PCR and screened for methicillin/oxacillin/cefoxitin resistance (MR) using the disk diffusion method and the PBP2' Latex Agglutination Test Kit. Enzyme production, hemolysis, DNase activity, slime production, and biofilm formation were tested in MRSA strains. The susceptibility of MRSA to eight partially-purified enterocins (Ent) produced by E. faecium and E. durans strains was checked using agar spot tests. The antibiofilm activity of Ents was tested using a quantitative plate assay. Out of 14 MRSA, PBP testing confirmed MR in 8 strains. The majority of MRSA showed DNase activity and β-hemolysis. Slime production and moderate biofilm formation were observed in all strains. MRSA were susceptible to tested Ents (100-12,800 AU/mL; except Ent4231). The antibiofilm effect of Ents (except Ent4231) was noted in the high range (64.9-97.0%). These results indicate that enterocins offer a promising option for the prevention and treatment of bacterial infections caused by biofilm-forming MRSA.
高毒力和多重耐药菌株(包括耐甲氧西林金黄色葡萄球菌 [MRSA])的发生率不断上升,是一个严重的问题,因为它们难以治疗。本研究旨在评估新型肠菌素(Ent)对从兔子中分离出的潜在致病性 MRSA 菌株的抗菌和抗生物膜作用。使用 PCR 鉴定葡萄球菌,并使用圆盘扩散法和 PBP2'乳胶凝集试剂盒筛选耐甲氧西林/苯唑西林/头孢西丁(MR)。测试了 MRSA 菌株的酶产生、溶血、DNase 活性、粘液产生和生物膜形成。使用琼脂斑点试验检查了 MRSA 对由屎肠球菌和肠球菌产生的八种部分纯化肠菌素(Ent)的敏感性。使用定量平板测定法测试了 Ents 的抗生物膜活性。在 14 株 MRSA 中,PBP 检测证实 8 株为 MR。大多数 MRSA 显示出 DNase 活性和β溶血。所有菌株均产生粘液并形成中度生物膜。MRSA 对测试的 Ents(100-12,800 AU/mL;除外 Ent4231)敏感。Ents(除外 Ent4231)的抗生物膜作用在高范围内(64.9-97.0%)。这些结果表明,肠菌素为预防和治疗由生物膜形成的 MRSA 引起的细菌感染提供了一个有希望的选择。