National Institute of Mental Health, Klecany, Czechia.
Third Faculty of Medicine, Charles University, Prague, Czechia.
Transl Psychiatry. 2021 Oct 2;11(1):506. doi: 10.1038/s41398-021-01603-4.
Serotonergic psychedelics are recently gaining a lot of attention as a potential treatment of several neuropsychiatric disorders. Broadband desynchronization of EEG activity and disconnection in humans have been repeatedly shown; however, translational data from animals are completely lacking. Therefore, the main aim of our study was to assess the effects of tryptamine and phenethylamine psychedelics (psilocin 4 mg/kg, LSD 0.2 mg/kg, mescaline 100 mg/kg, and DOB 5 mg/kg) on EEG in freely moving rats. A system consisting of 14 cortical EEG electrodes, co-registration of behavioral activity of animals with subsequent analysis only in segments corresponding to behavioral inactivity (resting-state-like EEG) was used in order to reach a high level of translational validity. Analyses of the mean power, topographic brain-mapping, and functional connectivity revealed that all of the psychedelics irrespective of the structural family induced overall and time-dependent global decrease/desynchronization of EEG activity and disconnection within 1-40 Hz. Major changes in activity were localized on the large areas of the frontal and sensorimotor cortex showing some subtle spatial patterns characterizing each substance. A rebound of occipital theta (4-8 Hz) activity was detected at later stages after treatment with mescaline and LSD. Connectivity analyses showed an overall decrease in global connectivity for both the components of cross-spectral and phase-lagged coherence. Since our results show almost identical effects to those known from human EEG/MEG studies, we conclude that our method has robust translational validity.
血清素能迷幻剂作为几种神经精神疾病的潜在治疗方法最近受到了广泛关注。在人类中已经反复显示出 EEG 活动的宽带去同步化和断开连接；然而，来自动物的转化数据完全缺失。因此，我们研究的主要目的是评估色胺和苯乙胺迷幻剂（裸盖菇素 4mg/kg、LSD 0.2mg/kg、麦司卡林 100mg/kg 和 DOB 5mg/kg）对自由活动大鼠 EEG 的影响。使用由 14 个皮质 EEG 电极组成的系统，将动物的行为活动与随后仅在与行为不活动相对应的片段（类似于静息状态的 EEG）中的分析进行共定位，以达到高转化有效性。平均功率分析、地形脑映射和功能连接分析表明，所有迷幻剂无论结构家族如何，都会在 1-40Hz 范围内诱导 EEG 活动的整体和时间依赖性的全局降低/去同步化和断开连接。活动的主要变化发生在额叶和感觉运动皮层的大面积区域，显示出每种物质的一些微妙的空间模式。在用麦司卡林和 LSD 治疗后，在后期检测到枕部θ（4-8Hz）活动的反弹。连接分析显示，对于交叉谱和相位滞后相干性的两个分量，整体连通性都降低了。由于我们的结果显示出与人类 EEG/MEG 研究中已知的几乎相同的效果，我们得出结论，我们的方法具有稳健的转化有效性。
