Purple Ross J, Gupta Rahul, Thomas Christopher W, Golden Caroline T, Palomero-Gallagher Nicola, Carhartt-Harris Robin, Froudist-Walsh Seán, Jones Matthew W
School of Physiology, Pharmacology and Neuroscience, University of Bristol, Biomedical Sciences Building, University Walk, Bristol, UK.
School of Engineering Mathematics and Technology, University of Bristol, Merchant Venturers Building, Bristol, UK.
Mol Psychiatry. 2025 Aug 26. doi: 10.1038/s41380-025-03182-y.
We quantify cellular- and circuit-resolution neural network dynamics following therapeutically relevant doses of the psychedelic psilocybin. Using chronically implanted Neuropixels probes, we recorded local field potentials (LFP) alongside action potentials from hundreds of neurons spanning infralimbic, prelimbic and cingulate subregions of the medial prefrontal cortex of freely-behaving adult rats. Psilocybin (0.3 mg/kg or 1 mg/kg i.p.) unmasked 100 Hz high frequency oscillations that were most pronounced within the infralimbic cortex, persisted for approximately 1 h post-injection and were accompanied by decreased net neuronal firing rates and reduced spike-train complexity. These acute effects were more prominent during resting behaviour than during performance of a sustained attention task. LFP 1-, 2- and 6-days post-psilocybin showed gradually-emerging increases in beta and low-gamma (20-60 Hz) power, specific to the infralimbic cortex. These findings reveal features of psychedelic action not readily detectable in human brain imaging, implicating infralimbic network oscillations as potential biomarkers of psychedelic-induced network plasticity over multi-day timescales.
我们对给予具有治疗相关性剂量的迷幻剂裸盖菇素后细胞分辨率和回路分辨率的神经网络动力学进行了量化。使用长期植入的神经像素探针，我们记录了自由行为成年大鼠内侧前额叶皮质的下缘、前边缘和扣带亚区域数百个神经元的局部场电位（LFP）以及动作电位。裸盖菇素（腹腔注射0.3mg/kg或1mg/kg）揭示了100Hz的高频振荡，这种振荡在下缘皮质内最为明显，在注射后持续约1小时，并伴有净神经元放电率降低和峰序列复杂性降低。这些急性效应在静息行为期间比在持续注意力任务执行期间更为突出。裸盖菇素注射后1天、2天和6天的LFP显示，下缘皮质特有的β和低γ（20 - 60Hz）功率逐渐增加。这些发现揭示了在人类脑成像中不易检测到的迷幻作用特征，表明下缘网络振荡作为多日时间尺度上迷幻剂诱导的网络可塑性的潜在生物标志物。
