中国孑遗树种[具体树种未给出]的20个新型EST-SSR标记的开发与特征分析
Development and characterization of 20 novel EST-SSR markers for , a relict tree in China.
作者信息
Zhang Mengyuan, Zhang Yunyan, Wang Guozheng, Zhou Jingbo, Tian Yongjing, Geng Qifang, Wang Zhongsheng
机构信息
College of Life Sciences Nanjing University Nanjing 210023 People's Republic of China.
出版信息
Appl Plant Sci. 2020 Feb 11;8(2):e11320. doi: 10.1002/aps3.11320. eCollection 2020 Feb.
PREMISE
(Ulmaceae), the only species of the genus , is an endangered tree in China. Here, novel expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed to illuminate its genetic diversity for conservation and assisted breeding.
METHODS AND RESULTS
Based on Illumina transcriptome data from , a total of 70 EST-SSR markers were initially designed and tested. Forty-eight of 70 loci (68.6%) were successfully amplified, of which 20 were polymorphic. The number of alleles per locus ranged from two to six, and the levels of observed and expected heterozygosity ranged from 0.018 to 0.781 and from 0.023 to 0.702, respectively. Additionally, cross-amplification was successful for 17 loci in two related species, and .
CONCLUSIONS
These new EST-SSR markers are valuable transcriptomic resources for and will facilitate population genetics and molecular breeding of this species and its relatives in Ulmaceae.
前提
（榆科），该属唯一的物种，是中国的一种濒危树木。在此，开发了新的表达序列标签 - 简单序列重复（EST - SSR）标记，以阐明其遗传多样性，用于保护和辅助育种。
方法与结果
基于来自的Illumina转录组数据，最初设计并测试了总共70个EST - SSR标记。70个位点中的48个（68.6%）成功扩增，其中20个具有多态性。每个位点的等位基因数从2到6不等，观察到的杂合度和预期杂合度水平分别从0.018到0.781和从0.023到0.702。此外，17个位点在两个相关物种和中成功进行了交叉扩增。
结论
这些新的EST - SSR标记是用于的有价值的转录组资源，将促进该物种及其榆科亲属的群体遗传学和分子育种。
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