Moreira Lara Elloyse Almeida, de Farias Cabral Vitória Pessoa, Rodrigues Daniel Sampaio, Barbosa Amanda Dias, Silveira Maria Janielly Castelo Branco, Coutinho Tatiana do Nascimento Paiva, Barbosa Sarah Alves, Sá Lívia Gurgel do Amaral Valente, de Andrade Neto João Batista, da Rocha Sania Nara Costa, Reis Celina Santos, Cavalcanti Bruno Coelho, Rios Maria Erivanda França, de Moraes Manoel Odorico, Júnior Hélio Vitoriano Nobre, da Silva Cecília Rocha
School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, Brazil.
Drug Research and Development Center, Federal University of Ceará, Fortaleza, Brazil.
Braz J Microbiol. 2024 Dec;55(4):3679-3690. doi: 10.1007/s42770-024-01477-w. Epub 2024 Aug 23.
The increase in fungal resistance is a major public health concern. In this context, Candida spp. is an important genus related to invasive diseases, especially in immunosuppressed patients. The relevance of alternative approaches to increasing fungal resistance stands out, in which products of natural origin demonstrate potential antifungal activity in vitro against Candida spp. In this sense, this work aimed to evaluate the in vitro activity of tannic acid against Candida spp. Minimum inhibitory concentration (MIC) was determined for tannic acid and the antifungals, and the checkerboard assay was performed to analyze the interactions between them. Furthermore, we evaluated the tannic acid antibiofilm activity and its possible mechanism of action. Tannic acid showed MIC ranging to 0.06 to 0.5 µg/ml and showed no loss of effectiveness when combined with antifungals. Also, is safe at the concentrations it exerts its antifungal activity in pre-formed biofilms, as demonstrated by IC50 in murine fibroblasts cells and the hemolytic assay. Additionally, its mechanisms of action can be related with induction of signals that lead to apoptosis in fungal cells.
真菌耐药性的增加是一个重大的公共卫生问题。在此背景下，念珠菌属是与侵袭性疾病相关的重要菌属，尤其是在免疫抑制患者中。增加真菌耐药性的替代方法的相关性尤为突出，其中天然来源的产品在体外对念珠菌属表现出潜在的抗真菌活性。从这个意义上说，这项工作旨在评估鞣酸对念珠菌属的体外活性。测定了鞣酸和抗真菌药物的最低抑菌浓度（MIC），并进行棋盘法分析它们之间的相互作用。此外，我们评估了鞣酸的抗生物膜活性及其可能的作用机制。鞣酸的MIC范围为0.06至0.5μg/ml，与抗真菌药物联合使用时未显示效力丧失。此外，如在小鼠成纤维细胞中的IC50和溶血试验所示，其在对预先形成的生物膜发挥抗真菌活性的浓度下是安全的。此外，其作用机制可能与诱导导致真菌细胞凋亡的信号有关。
