Antifungal Susceptibility and Biofilm Production of spp. Isolated from Clinical Samples.

OBJECTIVE

The study aims to speciate clinical isolates and detect their biofilm-forming ability and antifungal resistance.

METHODS

All the spp. isolated from different clinical samples like pus, urine, blood, and body fluid were included in the study. Biofilm production was tested by the microtiter plate method. Antifungal susceptibility was studied by the disk diffusion method. Patient's demographic details such as age, sex, and clinical information were collected. Presence of other risk factors such as diabetes mellitus, history of antibiotic use, and any urinary tract instrumentations was also recorded.

RESULTS

Among 90 species isolated, most predominant species was found to be (45.5%) followed by (28.88%), (20%), (3.33%), and (2.22%). spp. were isolated from urine (43%), BAL/sputum (18.88%), high vaginal swab (8.88%), suction tips (7.77%), blood and wound swabs (6.66%), pus (3.33%), bile aspirate (2.22%), and deep tissue (1.11%). A larger number of females were affected than males, and the age group of 51 to 60 years was more susceptible to candidiasis. A higher number of isolates produced biofilm followed by , , and However, showed no biofilm production in our study. All isolates were 100% sensitive to amphotericin B. Voriconazole was the next effective drug with 81.11% susceptibility. 24.44% of strains were resistant to fluconazole.

CONCLUSION

Speciation of isolates, detection of ability to form the biofilm, and monitoring of antifungal susceptibility testing are necessary for appropriate treatment.