Liu Yang, Wang Nijia, Chan Chiu-Wing, Lu Aiping, Yu Yuanyuan, Zhang Ge, Ren Kangning
Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong, SAR China.
Guangdong-Hong Kong Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery, Hong Kong, Hong Kong, SAR China.
Front Cell Dev Biol. 2021 Sep 17;9:730035. doi: 10.3389/fcell.2021.730035. eCollection 2021.
Aptamers are sequences of single-strand oligonucleotides (DNA or RNA) with potential binding capability to specific target molecules, which are increasingly used as agents for analysis, diagnosis, and medical treatment. Aptamers are generated by a selection method named systematic evolution of ligands by exponential enrichment (SELEX). Numerous SELEX methods have been developed for aptamer selections. However, the conventional SELEX methods still suffer from high labor intensity, low operation efficiency, and low success rate. Thus, the applications of aptamer with desired properties are limited. With their advantages of low cost, high speed, and upgraded extent of automation, microfluidic technologies have become promising tools for rapid and high throughput aptamer selection. This paper reviews current progresses of such microfluidic systems for aptamer selection. Comparisons of selection performances with discussions on principles, structure, operations, as well as advantages and limitations of various microfluidic-based aptamer selection methods are provided.
适配体是具有与特定靶分子潜在结合能力的单链寡核苷酸（DNA或RNA）序列，越来越多地用作分析、诊断和治疗的试剂。适配体通过一种名为指数富集配体系统进化（SELEX）的筛选方法产生。已经开发了许多用于适配体筛选的SELEX方法。然而，传统的SELEX方法仍然存在劳动强度高、操作效率低和成功率低的问题。因此，具有所需特性的适配体的应用受到限制。微流控技术具有成本低、速度快和自动化程度提高的优点，已成为快速和高通量适配体筛选的有前途的工具。本文综述了用于适配体筛选的此类微流控系统的当前进展。提供了各种基于微流控的适配体筛选方法的筛选性能比较，并讨论了其原理、结构、操作以及优缺点。
