Weems C W, Reynolds L P, Huie J M, Hoyer G L, Behrman H R
Prostaglandins. 1985 Feb;29(2):161-73. doi: 10.1016/0090-6980(85)90199-6.
Two studies were conducted to determine the effects of PGE1 or PGE2 on luteal function and binding of luteinizing hormone (LH) to luteal cell membranes in nonpregnant ewes. In Study I, ewes (n=5 per group) received an injection of vehicle (VEH) or 333 micrograms of PGE1 or PGE2 into the tissue surrounding the ovarian vascular pedicle (intrapedicle) on day 7 postestrus. Systemic progesterone concentrations of PGE1-treated ewes were greater (P less than 0.01) than those of VEH-treated ewes at 24 and 48 hr after injection. For PGE2-treated ewes, progesterone concentrations were greater (P less than 0.01) than for VEH-treated ewes only at 24 hr. Neither PGE1 nor PGE2 affected luteal weights or LH binding capacity at 48 hr. Treatment with PGE1, however, increased (P less than 0.10) endogenously bound LH at this time. In Study II, ewes (n=5 per group) received an intrapedicle injection of VEH, or 10 mg of PGE1 or PGE2 on day 8 postestrus. Systemic progesterone concentrations in PGE1-treated ewes were less (P less than 0.01) than for VEH-treated ewes at 24 hr, but by 72 hr were not different from those of VEH-treated ewes. For PGE2-treated ewes, systemic progesterone declined steadily to reach low values by 72 hr. Prostaglandin E2 had no effect on luteal binding of LH at 72 hr, whereas PGE1 increased (P less than 0.05) LH binding capacity and endogenously bound LH. Although PGE2 had no apparent affect on luteal binding of LH in these studies, PGE1 may enhance the function of ovine corpora lutea by stimulating an increase in their binding of LH and capacity to bind LH when the CL receives a luteolytic signal.
开展了两项研究，以确定前列腺素E1（PGE1）或前列腺素E2（PGE2）对未孕母羊黄体功能以及黄体生成素（LH）与黄体细胞膜结合的影响。在研究I中，母羊（每组n = 5）在发情期后第7天，在卵巢血管蒂周围组织（血管蒂内）注射赋形剂（VEH）或333微克PGE1或PGE2。注射后24小时和48小时，PGE1处理的母羊的全身孕酮浓度高于（P < 0.01）VEH处理的母羊。对于PGE2处理的母羊，孕酮浓度仅在24小时时高于（P < 0.01）VEH处理的母羊。在48小时时，PGE1和PGE2均未影响黄体重量或LH结合能力。然而，此时PGE1处理增加了（P < 0.10）内源性结合的LH。在研究II中，母羊（每组n = 5）在发情期后第8天接受血管蒂内注射VEH、10毫克PGE1或PGE2。PGE1处理的母羊在24小时时的全身孕酮浓度低于（P < 0.01）VEH处理的母羊，但到72小时时与VEH处理的母羊无差异。对于PGE2处理的母羊，全身孕酮在72小时时稳步下降至低值。前列腺素E2在72小时时对黄体LH结合无影响，而PGE1增加了（P < 0.05）LH结合能力和内源性结合的LH。尽管在这些研究中PGE2对黄体LH结合无明显影响，但当黄体接受溶黄体信号时，PGE1可能通过刺激黄体LH结合及其结合LH能力的增加来增强绵羊黄体的功能。
