Faculty of Medicine, Physiology Department, Cairo University, Giza, Egypt.
Faculty of Medicine, Histology and Cell Biology Department, Cairo University, Giza, Egypt.
PLoS One. 2021 Oct 5;16(10):e0258254. doi: 10.1371/journal.pone.0258254. eCollection 2021.
Epidemiology and pathogenesis of cardiovascular diseases (CVD) and osteoporosis are strikingly overlapping. This study presents matrix metalloproteinase-9 (MMP-9), as a simple molecular link more consistently associated with the pathophysiology of both osteoporosis and CVD risk factors. 40 adult female rats were randomly distributed into 4 groups [control sham-operated, untreated osteoporosis, carvedilol-treated osteoporosis and alendronate-treated osteoporosis]. After 8 weeks, blood samples were collected to estimate Lipid profile (Total cholesterol, HDL, Triglycerides), inflammatory markers (IL-6, TNF alpha, CRP and NO), and Bone turnover markers (BTM) (Alkaline phosphatase, osteocalcin and pyridinoline). The tibias were dissected to estimate MMP-9 and NF-kB gene expression, OPG, RANKL levels and for histological examination. Induction of osteoporosis resulted in a significant elevation in BTM, inflammatory markers and dyslipidemia. MMP-9 was significantly elevated and positively correlated with BTM, inflammation and dyslipidemia markers. Carvedilol and alendronate exerted a bone preservative role and attenuated dyslipidaemia and inflammation in accordance with their respective effect on MMP-9.
心血管疾病 (CVD) 和骨质疏松症的流行病学和发病机制惊人地重叠。本研究提出基质金属蛋白酶-9 (MMP-9)，作为一个与骨质疏松症和 CVD 危险因素的病理生理学更一致相关的简单分子联系。40 只成年雌性大鼠被随机分为 4 组 [对照组假手术、未治疗骨质疏松症、卡维地洛治疗骨质疏松症和阿仑膦酸钠治疗骨质疏松症]。8 周后，采集血样以估计血脂谱（总胆固醇、HDL、甘油三酯）、炎症标志物（IL-6、TNF alpha、CRP 和 NO）和骨转换标志物（BTM）（碱性磷酸酶、骨钙素和吡啶啉）。解剖胫骨以估计 MMP-9 和 NF-kB 基因表达、OPG、RANKL 水平并进行组织学检查。骨质疏松症的诱导导致 BTM、炎症标志物和血脂异常显著升高。MMP-9 显著升高，并与 BTM、炎症和血脂异常标志物呈正相关。卡维地洛和阿仑膦酸钠具有骨保护作用，并根据它们对 MMP-9 的各自作用减轻血脂异常和炎症。
