Zheng Xuefeng, Zhang Yuanyuan, Guo Shiming, Zhang Wenming, Wang Jinyun, Lin Yanping
Acupuncture College, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
Orthopedics Department, Zhangzhou Hospital of Traditional Chinese Medicine, Zhangzhou, Fujian 363000, P.R. China.
Exp Ther Med. 2018 Sep;16(3):1807-1813. doi: 10.3892/etm.2018.6356. Epub 2018 Jun 27.
The aim of the present study was to examine the dynamic expression of matrix metalloproteinase (MMP)-2, MMP-9 and MMP-13 in an ovariectomy (OVX)-induced osteoporosis rat model. A total of 80 Sprague-Dawley female rats (age, 3 months) were randomly divided into the OVX and sham groups, with 40 rats in each group. Rats in the sham group received sham surgery, while the remaining rats were ovariectomized. After 12, 16, 20 and 24 weeks, 10 rats from each group were randomly sacrificed, respectively. It was observed that the bone mineral density (BMD) and the trabecular bone area in the OVX group were significantly lower as compared with those in the sham group (P<0.01). The expression levels of MMP-2 and MMP-9 were negatively correlated with the BMD, while MMP-13 was positively correlated with the BMD. The expression levels of MMP-2 and MMP-9 increased more abruptly and were significant higher in the OVX group in comparison with those in the sham group between 12 and 24 weeks after surgery (P<0.01). More specifically, the MMP-9 mRNA expression level in the OVX group increased abruptly between 12 and 24 weeks after surgery. By contrast, in the sham group, the MMP-9 mRNA level was undetectable between 12 and 16 weeks, and increased steadily between 16 and 24 weeks. Furthermore, the mRNA and protein expression levels of MMP-13 initially increased and then decreased in the OVX group (P<0.01 vs. the sham group), whereas they continuously increased in the sham group between 12 and 24 weeks after surgery. In conclusion, MMP-2, MMP-9 and MMP-13 regulated the development of osteoporosis, and MMP-9 may be used as an important marker in the early diagnosis of osteoporosis.
本研究旨在检测基质金属蛋白酶（MMP）-2、MMP-9和MMP-13在卵巢切除（OVX）诱导的骨质疏松大鼠模型中的动态表达。总共80只3月龄的Sprague-Dawley雌性大鼠被随机分为OVX组和假手术组，每组40只。假手术组大鼠接受假手术，其余大鼠行卵巢切除术。术后12、16、20和24周时，分别从每组中随机处死10只大鼠。结果发现，与假手术组相比，OVX组的骨密度（BMD）和骨小梁面积显著降低（P<0.01）。MMP-2和MMP-9的表达水平与BMD呈负相关，而MMP-13与BMD呈正相关。术后12至24周，与假手术组相比，OVX组MMP-2和MMP-9的表达水平升高更为突然且显著更高（P<0.01）。更具体地说，OVX组中MMP-9 mRNA表达水平在术后12至24周突然升高。相比之下，在假手术组中，12至16周时未检测到MMP-9 mRNA水平，16至24周时稳步升高。此外，OVX组中MMP-13的mRNA和蛋白表达水平先升高后降低（与假手术组相比，P<0.01），而在假手术组中，术后12至24周其表达水平持续升高。总之，MMP-2、MMP-9和MMP-13调节骨质疏松的发展，MMP-9可能作为骨质疏松早期诊断的重要标志物。
